Scheller Frieder W, Bistolas Nikitas, Liu Songqin, Jänchen Michael, Katterle Martin, Wollenberger Ulla
University of Potsdam, Institute of Biochemistry and Biology, Department of Analytical Biochemistry, Karl-Liebknecht-Strasse 24-25, Bldg. 25, D 14476 Golm, Germany.
Adv Colloid Interface Sci. 2005 Nov 30;116(1-3):111-20. doi: 10.1016/j.cis.2005.05.006. Epub 2005 Aug 15.
Electrochemical investigations of the blood oxygen carrier protein include both mediated and direct electron transfer. The reaction of haemoglobin (Hb) with typical mediators, e.g., ferricyanide, can be quantified by measuring the produced ferrocyanide which is equivalent to the Hb concentration. Immobilization of the mediator within the electrode body allows reagentless electrochemical measuring of Hb. On the other hand, entrapment of the protein within layers of polyelectrolytes, lipids, nanoparticles of clay or gold leads to a fast heterogeneous electron exchange of the partially denatured Hb.
对血液氧载体蛋白的电化学研究包括介导电子转移和直接电子转移。血红蛋白（Hb）与典型的介质（如铁氰化物）的反应可以通过测量产生的亚铁氰化物来定量，亚铁氰化物的量与Hb浓度相当。将介质固定在电极体内可实现对Hb的无试剂电化学测量。另一方面，将蛋白质包埋在聚电解质、脂质、粘土或金的纳米颗粒层中会导致部分变性的Hb发生快速的异相电子交换。
