Fader Claudio M, Savina Ariel, Sánchez Diego, Colombo María I
Laboratorio de Biología Celular y Molecular, Instituto de Histología y Embriología (IHEM)-CONICET, Facultad de Ciencias Médicas, Universidad Nacional de Cuyo, Casilla de Correo 56, Centro Universitario, Parque General San Martín, 5500 Mendoza, Argentina.
Blood Cells Mol Dis. 2005 Sep-Oct;35(2):153-7. doi: 10.1016/j.bcmd.2005.07.002.
During reticulocyte maturation, some membrane proteins and organelles that are not required in the mature red cell are lost. These proteins are released into the extracellular medium associated with vesicles present in multivesicular bodies (MVBs). Fusion of MVBs with the plasma membrane results in secretion of the small internal vesicles, termed exosomes. By studying MVBs fusion and exosome release in K562 cells, a human erythroleukemic cell line, we have determined the functional significance of Rab11 and calcium in these events. Additionally, in the transformation process that occurs during erythrocyte maturation, intracellular organelles are likely removed as a consequence of autophagic sequestration and degradation. We propose K562 cells as a useful tool to analyze, at the molecular level, the role of autophagy in the terminal differentiation of red cells.
在网织红细胞成熟过程中，成熟红细胞中不需要的一些膜蛋白和细胞器会丢失。这些蛋白质会与多囊泡体（MVBs）中存在的囊泡一起释放到细胞外介质中。MVBs与质膜融合导致小的内部囊泡（即外泌体）的分泌。通过研究人红白血病细胞系K562细胞中的MVBs融合和外泌体释放，我们确定了Rab11和钙在这些事件中的功能意义。此外，在红细胞成熟过程中发生的转化过程中，细胞内细胞器可能由于自噬隔离和降解而被清除。我们提出将K562细胞作为一种有用的工具，在分子水平上分析自噬在红细胞终末分化中的作用。
