Fu Yu-Show, Cheng Yun-Chih, Lin Maan-Yuh Anya, Cheng Henrich, Chu Pei-Ming, Chou Shih-Chich, Shih Yang-Hsin, Ko Miau-Hwa, Sung Min-Shan
155 Sec. 2, Li-Nung Street, 112, Department of Anatomy, School of Medicine, National Yang-Ming University, Taipei, Taiwan.
Stem Cells. 2006 Jan;24(1):115-24. doi: 10.1634/stemcells.2005-0053. Epub 2005 Aug 11.
Human mesenchymal stem cells isolated from Wharton's jelly of the umbilical cord were induced to transform into dopaminergic neurons in vitro through stepwise culturing in neuron-conditioned medium, sonic hedgehog, and FGF8. The success rate was 12.7%, as characterized by positive staining for tyrosine hydroxylase (TH), the rate-limiting catecholaminergic synthesizing enzyme, and dopamine being released into the culture medium. Transplantation of such cells into the striatum of rats previously made Parkinsonian by unilateral striatal lesioning with the dopaminergic neurotoxin 6-hydroxydopamine partially corrected the lesion-induced amphetamine-evoked rotation. Viability of the transplanted cells at least 4 months after transplantation was identified by positive TH staining and migration of 1.4 mm both rostrally and caudally. These results suggest that human umbilical mesenchymal stem cells have the potential for treatment of Parkinson's disease.
从脐带华通氏胶中分离出的人骨髓间充质干细胞，通过在神经元条件培养基、音猬因子和FGF8中逐步培养，在体外被诱导转化为多巴胺能神经元。成功率为12.7%，其特征为酪氨酸羟化酶（TH）呈阳性染色，TH是儿茶酚胺能合成的限速酶，且多巴胺释放到培养基中。将这些细胞移植到先前通过用多巴胺能神经毒素6-羟基多巴胺进行单侧纹状体损伤而患帕金森病的大鼠纹状体中，可部分纠正损伤诱导的苯丙胺诱发的旋转。移植后至少4个月，通过TH阳性染色以及细胞向头侧和尾侧各迁移1.4毫米来确定移植细胞的活力。这些结果表明，人脐带间充质干细胞具有治疗帕金森病的潜力。
