Khan Z U, Ahmad S, Mokaddas E, Al-Sweih N, Chandy R
Department of Microbiology, Faculty of Medicine, Kuwait University, PO Box 24923, 13110, Kuwait.
Indian J Med Microbiol. 2005 Jul;23(3):182-5. doi: 10.4103/0255-0857.16605.
A sunflower (Helianthus annuus) seed husk agar medium has been developed and evaluated for differentiation of Candida dubliniensis from Candida albicans on the basis of colony morphology and chlamydospore production. All C. dubliniensis isolates (n=40) produced rough colonies with hyphal fringes and abundant chlamydospores whereas 101 of 105 (96.2%) C. albicans isolates produced smooth colonies with no evidence of chlamydospore production. Since this medium is free from oil droplets, chlamydospores can be examined with greater clarity by Dalmau plate technique. This medium provides a simple and cost-effective tool for the presumptive differentiation of C. dubliniensis from C. albicans and is particularly suited for clinical microbiology laboratories where biochemical or molecular methods for the differentiation of these two species are not available.
已开发并评估了一种向日葵（向日葵）种子壳琼脂培养基，用于根据菌落形态和厚垣孢子产生情况区分都柏林念珠菌和白色念珠菌。所有都柏林念珠菌分离株（n = 40）均产生带有菌丝边缘的粗糙菌落和大量厚垣孢子，而105株白色念珠菌分离株中的101株（96.2%）产生光滑菌落，没有厚垣孢子产生的迹象。由于该培养基没有油滴，因此可以通过达尔 Mau 平板技术更清晰地检查厚垣孢子。该培养基为从白色念珠菌中初步区分都柏林念珠菌提供了一种简单且经济高效的工具，特别适用于无法使用生化或分子方法区分这两个菌种的临床微生物实验室。
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