Jeong HoeSu, Rhim TaiYoun, Ahn Mi-Hyun, Yoon Pyoung-Oh, Kim Sung-Ho, Chung Il Yup, Uh SooTaek, Kim Sung-Il, Park Choon-Sik
Genome Research Center for Allergy and Respiratory Diseases, Soonchunhyang University Hospital, Korea.
J Korean Med Sci. 2005 Aug;20(4):579-85. doi: 10.3346/jkms.2005.20.4.579.
Allergic asthma is associated with persistent functional and structural changes in the airways and involves many different cell types. Many proteins involved in allergic asthma have been identified individually, but complete protein profiles (proteome) have not yet been reported. Here we have used a differential proteome mapping strategy to identify tissue proteins that are differentially expressed in mice with allergic asthma and in normal mice. Mouse lung tissue proteins were separated using two-dimensional gel electrophoresis over a pH range between 4 and 7, digested, and then analyzed by matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MS). The proteins were identified using automated MS data acquisition. The resulting data were searched against a protein database using an internal Mascot search routine. This approach identified 15 proteins that were differentially expressed in the lungs of mice with allergic asthma and normal mice. All 15 proteins were identified by MS, and 9 could be linked to asthma-related symptoms, oxidation, or tissue remodeling. Our data suggest that these proteins may prove useful as surrogate biomarkers for quantitatively monitoring disease state progression or response to therapy.
过敏性哮喘与气道持续的功能和结构改变相关，且涉及多种不同细胞类型。许多参与过敏性哮喘的蛋白质已被逐一鉴定，但完整的蛋白质谱（蛋白质组）尚未见报道。在此，我们采用差异蛋白质组图谱策略来鉴定在过敏性哮喘小鼠和正常小鼠中差异表达的组织蛋白质。小鼠肺组织蛋白质在pH值4至7的范围内通过二维凝胶电泳进行分离、消化，然后通过基质辅助激光解吸/电离飞行时间质谱（MS）进行分析。使用自动MS数据采集来鉴定蛋白质。所得数据使用内部Mascot搜索程序在蛋白质数据库中进行搜索。该方法鉴定出15种在过敏性哮喘小鼠和正常小鼠肺中差异表达的蛋白质。所有15种蛋白质均通过MS鉴定，其中9种可与哮喘相关症状、氧化或组织重塑相关联。我们的数据表明，这些蛋白质可能作为替代生物标志物，用于定量监测疾病状态进展或对治疗的反应，具有一定作用。
