Satozono Hiroshi, Kazumura Kimiko, Okazaki Sigetoshi, Hiramatsu Mitsuo
Hamamatsu Photonics K.K., 5000 Hirakuchi, Hamamatsu-City 434-8601, Japan.
Luminescence. 2006 Mar-Apr;21(2):69-71. doi: 10.1002/bio.882.
We have developed a novel method for simultaneously measuring fluorescence and chemiluminescence. The generation of superoxide anion and the intracellular Ca(2+) ion concentration of neutrophil-like cells stimulated by agonists were measured in real time by our method. Our results were in agreement with the intracellular signalling in the neutrophils. We also found that the presence of Zn(2+) ion inhibited both the generation of superoxide anion and the influx of Ca(2+) ions.
我们开发了一种同时测量荧光和化学发光的新方法。通过我们的方法实时测量了激动剂刺激的类中性粒细胞中超氧阴离子的产生和细胞内Ca(2+)离子浓度。我们的结果与中性粒细胞中的细胞内信号传导一致。我们还发现Zn(2+)离子的存在抑制了超氧阴离子的产生和Ca(2+)离子的内流。
