Yagi-Chaves Sachiko N, Liu Gang, Yamashita Koichi, Manabe Masanobu, Song Shao-Jiang, Kodama Hiroyuki
Department of Anesthesiology and Critical Care Medicine, Kochi Medical School, Nankoku-shi, Kochi 783-8505, Japan.
Arch Biochem Biophys. 2006 Feb 1;446(1):84-90. doi: 10.1016/j.abb.2005.11.006.
Five triterpenoids; Congmuyanoside V, X, XI, XII, and XV (Cong. V, X, XI, XII, and XV) were isolated from the root bark of Aralia elata. The effect of these triterpenoids on stimulus-induced superoxide generation in human neutrophils was assayed by measuring the reduction of ferricytochrome c (cyt. c) using a dual-beam spectrophotometer. Translocation of p47(phox), p67(phox), and rac to the cell membrane and tyrosyl or serine/threonine phosphorylation of neutrophil proteins were investigated using specific monoclonal antibodies. The five triterpenoids used in the present experiment significantly suppressed N-formyl-methionyl-leucyl-phenylalanine (fMLP)-induced superoxide generation in concentration-dependent manner. Congmuyanoside V, XII, and XV also suppressed arachidonic acid (AA)-induced superoxide generation in high concentration. However, these triterpenoids showed no effect on phorbol 12-myristate 13-acetate (PMA)-induced superoxide generation. fMLP- and AA-induced tyrosyl phosphorylation and translocation of the cytosolic proteins p47(phox), p67(phox), and rac to the cell membrane were suppressed in parallel with the suppression of the stimulus-induced superoxide generation.
从辽东楤木根皮中分离出5种三萜类化合物：葱木皂苷V、X、XI、XII和XV（Cong. V、X、XI、XII和XV）。使用双光束分光光度计通过测量高铁细胞色素c（cyt. c）的还原情况，测定了这些三萜类化合物对人中性粒细胞中刺激诱导的超氧化物生成的影响。使用特异性单克隆抗体研究了p47(phox)、p67(phox)和rac向细胞膜的转位以及中性粒细胞蛋白的酪氨酸或丝氨酸/苏氨酸磷酸化。本实验中使用的5种三萜类化合物以浓度依赖性方式显著抑制了N-甲酰甲硫氨酰-亮氨酰-苯丙氨酸（fMLP）诱导的超氧化物生成。葱木皂苷V、XII和XV在高浓度时也抑制了花生四烯酸（AA）诱导的超氧化物生成。然而，这些三萜类化合物对佛波酯12-肉豆蔻酸酯13-乙酸酯（PMA）诱导的超氧化物生成没有影响。fMLP和AA诱导的酪氨酸磷酸化以及胞质蛋白p47(phox)、p67(phox)和rac向细胞膜的转位与刺激诱导的超氧化物生成的抑制同时受到抑制。
