Whiting M, Crichlow M, Ingledew W M, Ziola B
Department of Microbiology, College of Medicine, University of Saskatchewan, Saskatoon, Canada.
Appl Environ Microbiol. 1992 Feb;58(2):713-6. doi: 10.1128/aem.58.2.713-716.1992.
A membrane immunofluorescent-antibody test was developed to detect diacetyl-producing Pediococcus contaminants in brewery pitching yeast (yeast [Saccharomyces cerevisiae] slurry collected for reinoculation). Centrifugations at 11 and 5,100 x g separate yeast cells from bacteria and concentrate the bacteria, respectively. Pelleted bacteria resuspended and trapped on a black membrane filter are reacted with monoclonal antibodies specific for cell surface antigens and then with fluorescein-conjugated indicator antibodies. Whether pitching yeast is contaminated with pediococci at 0.001% is determined in less than 4 h. The sensitivity of the assay is 2 orders of magnitude below the Pediococcus detection limit of direct microscopy.
开发了一种膜免疫荧光抗体试验，用于检测啤酒厂接种酵母（收集用于再接种的酵母[酿酒酵母]浆液）中产生双乙酰的片球菌污染物。分别以11和5100×g离心可将酵母细胞与细菌分离并浓缩细菌。重悬并捕获在黑色膜过滤器上的沉淀细菌与针对细胞表面抗原的单克隆抗体反应，然后与荧光素偶联的指示抗体反应。在不到4小时内即可确定接种酵母是否被0.001%的片球菌污染。该检测方法的灵敏度比直接显微镜检测片球菌的检测限低2个数量级。
