Willingham M C, Pastan I
Ultrastructural Cytochemistry Section, National Cancer Institute, Bethesda, MD 20892.
Biotechniques. 1990 Mar;8(3):320-4.
Immunofluorescence microscopy is a powerful technique for detecting the location of surface and intracellular antigens in individual cells. However, using standard methods, processing large numbers of samples for immunofluorescence is cumbersome and difficult. To simplify greatly this process, we have developed a chamber that reversibly creates multiple small wells in a large (150 mm) tissue culture dish. This device allows the rapid and convenient processing of hundreds of samples each of 100 microliters volume. Each sample is examined using a short working distance, high numerical aperture immersion objective for maximum sensitivity and resolution. This apparatus makes immunofluorescence a practical method for the primary screening of hybridoma clones.
免疫荧光显微镜检查是一种用于检测单个细胞表面和细胞内抗原位置的强大技术。然而，使用标准方法对大量样本进行免疫荧光处理既繁琐又困难。为了极大地简化这一过程，我们开发了一种小室，它能在一个大的（150毫米）组织培养皿中可逆地创建多个小阱。该装置允许快速方便地处理数百个体积为100微升的样本。使用短工作距离、高数值孔径的浸液物镜对每个样本进行检测，以实现最大灵敏度和分辨率。该仪器使免疫荧光成为杂交瘤克隆初步筛选的实用方法。
