Warner Karen L, Larkin Michael J, Harper David B, Murrell J Colin, McDonald Ian R
Department of Biological Sciences, University of Warwick, Coventry CV4 7AL, UK.
FEMS Microbiol Lett. 2005 Oct 1;251(1):45-51. doi: 10.1016/j.femsle.2005.07.021.
Aminobacter lissarensis CC495 is an aerobic facultative methylotroph capable of growth on glucose, glycerol, pyruvate and methylamine as well as the methyl halides methyl chloride and methyl bromide. Previously, cells grown on methyl chloride have been shown to express two polypeptides with apparent molecular masses of 67 and 29 kDa. The 67 kDa protein was purified and identified as a halomethane:bisulfide/halide ion methyltransferase. This study describes a single gene cluster in A. lissarensis CC495 containing the methyl halide utilisation genes cmuB, cmuA, cmuC, orf 188, paaE and hutI. The genes correspond to the same order and have a high similarity to a gene cluster found in Aminobacter ciceronei IMB-1 and Hyphomicrobium chloromethanicum strain CM2 indicating that genes encoding methyl halide degradation are highly conserved in these strains.
利萨胺基杆菌CC495是一种兼性好氧甲基营养菌，能够利用葡萄糖、甘油、丙酮酸、甲胺以及卤代甲烷（如氯甲烷和溴甲烷）进行生长。此前研究表明，在氯甲烷上生长的细胞会表达两种表观分子量分别为67 kDa和29 kDa的多肽。67 kDa的蛋白质被纯化并鉴定为卤代甲烷：二硫化物/卤离子甲基转移酶。本研究描述了利萨胺基杆菌CC495中的一个单基因簇，其中包含卤代甲烷利用基因cmuB、cmuA、cmuC、orf 188、paaE和hutI。这些基因排列顺序相同，并且与在西塞罗胺基杆菌IMB - 1和氯甲烷生丝微菌CM2菌株中发现的一个基因簇高度相似，这表明编码卤代甲烷降解的基因在这些菌株中高度保守。
