Morabito Fortunato, Tomaino Antonio, Cristani Mariateresa, Cimino Francesco, Martino Massimo, Minciullo Paola Lucia, Calabrò Concetta, Saija Antonella, Gangemi Sebastiano
Bone Marrow Transplant Unit, Azienda Ospedaliera, Bianchi-Melacrino-Morelli, 89100 Reggio Calabria, Italy.
Transfus Apher Sci. 2005 Oct;33(2):141-6. doi: 10.1016/j.transci.2005.05.003.
Granulocyte-colony stimulating factor (G-CSF), the most established agent for the mobilization of stem cells in current clinical practice, could induce a condition of oxidative stress. Herein plasmatic levels of protein carbonyl groups (a biomarker of oxidative stress) were measured in a group of donors given recombinant human G-CSF (rHuG-CSF) at different times: (1) before starting rHuG-CSF administration, (2) on day 5 of rHuG-CSF administration, (3) on the same day immediately after the end of the first leukapheresis procedure and (4) one week after rHuG-CSF withdrawal. Plasma levels of protein carbonyl groups enhanced significantly in donors after 5 days of rHuG-CSF treatment and appeared further slightly increased following leukapheresis procedure; 7 days following rHuG-CSF withdrawal, they showed a tendency to normalization. These findings may contribute to better understand the oxidative reactions following rHuG-CSF treatment and leukapheresis.
粒细胞集落刺激因子（G-CSF）是目前临床实践中最常用的动员干细胞的药物，它可引发氧化应激状态。在此，我们对一组在不同时间给予重组人G-CSF（rHuG-CSF）的供体的血浆蛋白羰基水平（氧化应激的生物标志物）进行了测定：（1）开始给予rHuG-CSF之前；（2）给予rHuG-CSF第5天；（3）第一次白细胞分离术结束当天；（4）停用rHuG-CSF一周后。rHuG-CSF治疗5天后，供体血浆蛋白羰基水平显著升高，白细胞分离术后进一步略有升高；停用rHuG-CSF 7天后，它们呈现出恢复正常的趋势。这些发现可能有助于更好地理解rHuG-CSF治疗和白细胞分离术后的氧化反应。
