Hurst Suzanne Maria, Lyall K A, Hurst R D, Stevenson L M
Functional Food and Health Group, The New Zealand Institute of Plant and Food Research Ltd., Private Bag 3123, East Street, Hamilton, New Zealand.
Eur J Appl Physiol. 2009 Sep;107(1):61-72. doi: 10.1007/s00421-009-1099-1. Epub 2009 Jun 7.
Prolonged oxidative stress is detrimental to health; however, transient oxidative stress may improve immune capability. We examined whether exercise-induced increases in the plasma oxidative generating capability enhance immune responsiveness to potential pathogens. Twelve individuals underwent a 30-min row and pre and post-exercise bloods were collected for oxidative stress and immune assessment. We found that exercise induced a transient increase in plasma carbonyls (3.2-5.3 nmol/mg protein) and creatine kinase activity (0.5-1.2 absorbance/min/mg protein) and that lipopolysaccharide (LPS) stimulation (0.5-24 h) of pre- and post-exercise blood augmented temporal tumour necrosis factor-alpha (TNFalpha) secretion. Further characterisation of plasma using a modified dihydro-2',7'-dichlorohydrofluorescein (DCF) assay revealed that addition of a sub-threshold of hydrogen peroxide to post-exercise (and not pre-exercise) plasma caused a sixfold increase in the radical oxygen species (ROS) generating capability after 15 min (555 +/- 131 to 3607 +/- 488 change in fluorescent intensity [DeltaFI]), which was inhibited using 60 mM N-acetyl-L: -cysteine (920 +/- 154 DeltaFI). Furthermore, cell experiments revealed that LPS stimulation of either THP-1 cells pre-incubated with post-exercise plasma or peripheral blood mononuclear cells pre-treated with pro-oxidants, modulated the temporal secretion of key cytokines that regulate the initiation, progression and resolution of an inflammatory response. These results indicate that exercise-induced changes in plasma parameters (e.g. oxidative generating capability-dependent or independent of inflammatory mediators) augment the temporal LPS response and support the notion that repeated transient oxidative stress (such as that induced by regular exercise) is important for a "healthy" immune system.
长期的氧化应激对健康有害；然而，短暂的氧化应激可能会提高免疫能力。我们研究了运动引起的血浆氧化生成能力的增加是否会增强对潜在病原体的免疫反应性。12名个体进行了30分钟的划船运动，并在运动前后采集血液进行氧化应激和免疫评估。我们发现运动导致血浆羰基含量（3.2 - 5.3 nmol/mg蛋白质）和肌酸激酶活性（0.5 - 1.2吸光度/分钟/毫克蛋白质）短暂增加，并且运动前后血液的脂多糖（LPS）刺激（0.5 - 24小时）增强了肿瘤坏死因子-α（TNFα）的分泌。使用改良的二氢-2',7'-二氯荧光素（DCF）测定法对血浆进行进一步表征发现，向运动后（而非运动前）血浆中添加亚阈值的过氧化氢会导致15分钟后活性氧（ROS）生成能力增加六倍（荧光强度变化[ΔFI]从555±131变为3607±488），而使用60 mM N-乙酰-L-半胱氨酸可抑制此变化（920±154 ΔFI）。此外，细胞实验表明，用运动后血浆预孵育的THP-1细胞或用促氧化剂预处理的外周血单个核细胞经LPS刺激后，可调节关键细胞因子的分泌时间，这些细胞因子调节炎症反应的起始、进展和消退。这些结果表明，运动引起的血浆参数变化（例如依赖或独立于炎症介质的氧化生成能力）增强了对LPS的反应时间，并支持了反复短暂氧化应激（如规律运动诱导的氧化应激）对“健康”免疫系统很重要这一观点。
