[丙烯腈对大鼠睾丸抗氧化酶活性及脂质过氧化水平的影响]

Huang Jian-shu, Zhong Xian-jiu, Wu Xin, Jin Tai-yi

Jinshan Hospital of Fudan University, Shanghai 200540, China.

Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi. 2005 Apr;23(2):136-8.

OBJECTIVE

To explore the mechanism of male reproductive toxicity induced by acrylonitrile (ACN).

METHODS

Male Sprague-Dawley rats were daily administrated ACN by intraperitoneal injection 5 times a week for 13 weeks at the dose of 0, 7.5, 15.0 and 30.0 mg/kg body weight, respectively. The rats were sacrificed and testes were removed at the end of 4, 8, 13 or 15 weeks, respectively. The activities of glutathione peroxidase (GSH-Px), superoxide dismutase (SOD) and glutathione S-transferase (GST) and the levels of glutathione (GSH) and malonaldehyde (MDA) were detected in testes.

RESULTS

Following ACN treatment of 4 weeks, the levels of GSH in ACN 15.0 mg/kg and 30.0 mg/kg group were (7.44 +/- 0.77) mg/g pro and (6.95 +/- 0.77) mg/g pro respectively, and the activity of GSH-Px was (70.89 +/- 4.01) U/mg pro in 30.0 mg/kg group, all of which were significantly higher than the control group (P < 0.05, P < 0.01). After 8 weeks, the levels of GSH decreased to (2.50 +/- 0.94) mg/g pro in ACN 30.0 mg/kg group (P < 0.01); the activities of SOD increased to (102.08 +/- 16.08) NU/mg pro and (113.30 +/- 17.20) NU/mg pro in ACN 15.0 mg/kg and 30.0 mg/kg group (P < 0.01). After 13 weeks, the levels of GSH declined in ACN 15.0 mg/kg and 30.0 mg/kg group, and the activities of GST decreased in ACN 30.0 mg/kg group, and of GSH-Px decreased in both doses group. However, the level of MDA [(0.68 +/- 0.16) nmol/mg pro] were significantly higher in 30.0 mg/kg group than that in control group [(0.38 +/- 0.12) nmol/mg pro] (P < 0.01). 2 weeks after stopping ACN treatment, the level of GSH restored to normal but the levels of MDA or the activity of GSH-Px in 30.0 mg/kg group were still higher or lower respectively than those of control (P < 0.05).

CONCLUSION

ACN may impair the balance of antioxidant system, thus induce lipid peroxidation damage to rat testes.

目的

探讨丙烯腈（ACN）诱导雄性生殖毒性的机制。

方法

将雄性Sprague-Dawley大鼠按体重分别以0、7.5、15.0和30.0 mg/kg的剂量，每周5次腹腔注射ACN，持续13周。分别在第4、8、13或15周结束时处死大鼠并取出睾丸。检测睾丸中谷胱甘肽过氧化物酶（GSH-Px）、超氧化物歧化酶（SOD）和谷胱甘肽S-转移酶（GST）的活性以及谷胱甘肽（GSH）和丙二醛（MDA）的水平。

结果

ACN处理4周后，ACN 15.0 mg/kg组和30.0 mg/kg组的GSH水平分别为（7.44±0.77）mg/g蛋白和（6.95±0.77）mg/g蛋白，30.0 mg/kg组的GSH-Px活性为（70.89±4.01）U/mg蛋白，均显著高于对照组（P<0.05，P<0.01）。8周后，ACN 30.0 mg/kg组的GSH水平降至（2.50±0.94）mg/g蛋白（P<0.01）；ACN 15.0 mg/kg组和30.0 mg/kg组的SOD活性分别升至（102.08±16.08）NU/mg蛋白和（113.30±17.20）NU/mg蛋白（P<0.01）。13周后，ACN 15.0 mg/kg组和30.0 mg/kg组的GSH水平下降，ACN 30.0 mg/kg组的GST活性下降，两个剂量组的GSH-Px活性均下降。然而，30.0 mg/kg组的MDA水平[（0.68±0.16）nmol/mg蛋白]显著高于对照组[（0.38±0.12）nmol/mg蛋白]（P<0.01）。停止ACN处理2周后，GSH水平恢复正常，但30.0 mg/kg组的MDA水平或GSH-Px活性仍分别高于或低于对照组（P<0.05）。