Armagan Abdullah, Uz Efkan, Yilmaz H Ramazan, Soyupek Sedat, Oksay Taylan, Ozcelik Nurten
Department of Urology, Suleyman Demirel University, Faculty of Medicine, Isparta 32050, Turkey.
Asian J Androl. 2006 Sep;8(5):595-600. doi: 10.1111/j.1745-7262.2006.00177.x. Epub 2006 Jun 5.
To examine the effects of melatonin treatment on lipid peroxidation (LPO) and the activities of antioxidant enzymes in the testicular tissue of streptozotocin (STZ)-induced diabetic rats.
Twenty-six male rats were randomly divided into three groups as follows: group I, control, non-diabetic rats (n = 9); group II, STZ-induced, untreated diabetic rats (n = 8); group III, STZ-induced, melatonin-treated (dose of 10 mg/kg . day) diabetic rats (n = 9). Following 8-week melatonin treatment, all rats were anaesthetized and then were killed to remove testes from the scrotum.
As compared to group I, in rat testicular tissues of group II , increased levels of malondialdehyde (MDA) (P < 0.01) and superoxide dismutase (SOD) (P < 0.01) as well as decreased levels of catalase (CAT) (P < 0.01) and glutathione peroxidase (GSH-Px) (P > 0.05) were found. In contrast, as compared to group II, in rat testicular tissues of group III, levels of MDA decreased (but this decrease was not significant, P > 0.05) and SOD (P < 0.01) as well as CAT (P < 0.05) increased. GSH-Px was not influenced by any of the treatment. Melatonin did not significantly affect the elevated glucose concentration of diabetic group. At the end of the study, there was no significant difference between the melatonin-treated group and the untreated group by means of body and testicular weight.
Diabetes mellitus increases oxidative stress and melatonin inhibits lipid peroxidation and might regulate the activities of antioxidant enzymes of diabetic rat testes.
研究褪黑素对链脲佐菌素（STZ）诱导的糖尿病大鼠睾丸组织脂质过氧化（LPO）及抗氧化酶活性的影响。
将26只雄性大鼠随机分为三组：第一组为对照组，非糖尿病大鼠（n = 9）；第二组为STZ诱导的未治疗糖尿病大鼠（n = 8）；第三组为STZ诱导的褪黑素治疗（剂量为10 mg/kg·天）糖尿病大鼠（n = 9）。经过8周的褪黑素治疗后，所有大鼠麻醉后处死，从阴囊中取出睾丸。
与第一组相比，第二组大鼠睾丸组织中丙二醛（MDA）水平升高（P < 0.01）、超氧化物歧化酶（SOD）水平升高（P < 0.01），而过氧化氢酶（CAT）水平降低（P < 0.01）、谷胱甘肽过氧化物酶（GSH-Px）水平降低（P > 0.05）。相反，与第二组相比，第三组大鼠睾丸组织中MDA水平降低（但降低不显著，P > 0.05），SOD（P < 0.01）和CAT（P < 0.05）水平升高。GSH-Px不受任何处理的影响。褪黑素对糖尿病组升高的血糖浓度无显著影响。研究结束时，褪黑素治疗组与未治疗组在体重和睾丸重量方面无显著差异。
糖尿病会增加氧化应激，褪黑素可抑制脂质过氧化，并可能调节糖尿病大鼠睾丸的抗氧化酶活性。
