Brunelle Alain, Touboul David, Laprévote Olivier
Laboratoire de Spectrométrie de Masse, Institut de Chimie des Substances Naturelles, CNRS, UPR 2301, Av. de la Terrasse, F91198, Gif- sur- Yvette Cedex, France.
J Mass Spectrom. 2005 Aug;40(8):985-99. doi: 10.1002/jms.902.
Time-of-flight secondary ion mass spectrometry (TOF-SIMS) using liquid metal ion guns (LMIGs) is now sensitive enough to produce molecular-ion images directly from biological tissue samples. Primary cluster ions strike a spot on the sample to produce a mass spectrum. An image of this sample is achieved by rastering the irradiated point over the sample surface. The use of secondary ion mass spectrometry for mapping biological tissue surfaces provides unique analytical capabilities; in particular, it enables in a single acquisition a large variety of biological compounds to be localised on a micrometer scale and scrutinised for colocalisations. Without any treatment of the sample, this method is fully compatible with subsequent and complementary analyses like fluorescence microscopy, histochemical staining, or even matrix-assisted laser desorption/ionisation imaging. Basic physical concepts, required instrumentation (ion source and mass analyzer), sample preparation methods, image acquisition, image processing, and emerging biological applications will be described and discussed.
使用液态金属离子枪（LMIG）的飞行时间二次离子质谱（TOF-SIMS）现在灵敏度足够高，能够直接从生物组织样本中生成分子离子图像。初级簇离子撞击样本上的一个点以产生质谱。通过在样本表面扫描照射点来获得该样本的图像。将二次离子质谱用于绘制生物组织表面图谱具有独特的分析能力；特别是，它能够在一次采集中将多种生物化合物定位在微米尺度上，并对共定位情况进行仔细检查。无需对样本进行任何处理，该方法与后续的荧光显微镜、组织化学染色甚至基质辅助激光解吸/电离成像等补充分析完全兼容。将描述和讨论基本物理概念、所需仪器（离子源和质量分析器）、样本制备方法、图像采集、图像处理以及新兴的生物应用。
