Lan Yunpeng, Zou Zhu, Yang Zhibo
Department of Chemistry and Biochemistry, University of Oklahoma, Norman, OK, 73019, USA.
Trends Analyt Chem. 2024 May;174. doi: 10.1016/j.trac.2024.117657. Epub 2024 Mar 19.
Studying cell heterogeneity can provide a deeper understanding of biological activities, but appropriate studies cannot be performed using traditional bulk analysis methods. The development of diverse single cell bioanalysis methods is in urgent need and of great significance. Mass spectrometry (MS) has been recognized as a powerful technique for bioanalysis for its high sensitivity, wide applicability, label-free detection, and capability for quantitative analysis. In this review, the general development of single cell mass spectrometry (SCMS) field is covered. First, multiple existing SCMS techniques are described and compared. Next, the development of SCMS field is discussed in a chronological order. Last, the latest quantification studies on small molecules using SCMS have been described in detail.
研究细胞异质性有助于更深入地理解生物活性，但传统的整体分析方法无法进行适当的研究。因此，迫切需要开发各种单细胞生物分析方法，这具有重要意义。质谱（MS）因其高灵敏度、广泛适用性、无标记检测和定量分析能力，已被公认为一种强大的生物分析技术。在这篇综述中，涵盖了单细胞质谱（SCMS）领域的总体发展情况。首先，描述并比较了多种现有的SCMS技术。其次，按时间顺序讨论了SCMS领域的发展。最后，详细介绍了使用SCMS对小分子进行的最新定量研究。
