Weekes E W, Tamura T, Davis R O, Birch R, Vaughn W H, Franklin J C, Barganier C, Cosper P, Finley S C, Finley W H
Department of Nutrition Sciences, University of Alabama, Birmingham 35294.
Biol Neonate. 1992;61(4):226-31. doi: 10.1159/000243747.
We analyzed nutrient levels in amniotic fluid obtained during the second trimester of normal, uncomplicated pregnancies from 221 women who delivered apparently healthy infants and from 8 with neural tube defect (NTD) pregnancies. Folate was measured by microbiological assay, vitamin B12 by a radiobinding method, and zinc, copper and iron by atomic absorption spectrophotometry. We found that the mean amniotic fluid nutrient levels of normal pregnancies were 24.7 nmol/l for folate, 600 pmol/l for vitamin B12, and 1.7, 1.9, and 9.0 mumol/l for zinc, copper and iron, respectively. Amniotic fluid folate, zinc, copper and iron levels of NTD pregnancies were similar to those found during normal pregnancy, however, vitamin B12 levels were markedly lower than those of normal pregnancies.
我们分析了221名分娩出健康婴儿的正常、无并发症孕妇以及8名神经管缺陷（NTD）孕妇在妊娠中期获取的羊水样本中的营养物质水平。叶酸通过微生物测定法进行测量，维生素B12采用放射结合法，锌、铜和铁则通过原子吸收分光光度法进行测定。我们发现，正常妊娠的羊水平均营养物质水平分别为：叶酸24.7纳摩尔/升、维生素B12 600皮摩尔/升、锌1.7微摩尔/升、铜1.9微摩尔/升以及铁9.0微摩尔/升。神经管缺陷妊娠的羊水叶酸、锌、铜和铁水平与正常妊娠时相似，然而，维生素B12水平明显低于正常妊娠。
