Vrouwenvelder W C, Groot C G, de Groot K
Department of Biomaterials, School of Medicine, University of Leiden, The Netherlands.
Biomaterials. 1992;13(6):382-92. doi: 10.1016/0142-9612(92)90044-o.
We examined the behaviour of fetal rat osteoblasts cultured upon bioactive glass and nonreactive glasses, and the supposed stimulatory effects of bioactive glass on osteoblasts. Nonreactive glass cultures showed flattened cells with almost no dorsal ruffles. Bioactive glass cultures showed compact cells with dorsal ruffles and filapodia resulting in the formation of a denser cell layer. For confluent nonreactive glass cultures the osteoblast expression was mainly concentrated in the clustered cells which were formed upon the monolayer, whereas for confluent bioactive glass cultures the osteoblast expression was more generally distributed. The production of type I collagen, osteocalcin and an osteoblast-specific antigen was shown by immunocytochemistry for all cultures, although differences in distribution were observed. The bioactive layer of bioactive glass is responsible for a better osteoblast-like morphology, a higher proliferation rate and generally a better osteoblast expression.
我们研究了在生物活性玻璃和非活性玻璃上培养的胎鼠成骨细胞的行为，以及生物活性玻璃对成骨细胞的假定刺激作用。非活性玻璃培养的细胞扁平，几乎没有背侧褶皱。生物活性玻璃培养的细胞紧密，有背侧褶皱和丝状伪足，形成了更密集的细胞层。对于汇合的非活性玻璃培养物，成骨细胞表达主要集中在单层上形成的聚集细胞中，而对于汇合的生物活性玻璃培养物，成骨细胞表达分布更普遍。免疫细胞化学显示，所有培养物都产生I型胶原蛋白、骨钙素和成骨细胞特异性抗原，尽管观察到分布存在差异。生物活性玻璃的生物活性层有助于形成更好的成骨细胞样形态、更高的增殖率以及总体上更好的成骨细胞表达。
