Gao Yuan, Cao Wen-Ling, Wang Xiao-Yan, Gong Yan-Dao, Tian Jie-Mo, Zhao Nan-Ming, Zhang Xiu-Fang
Department of Biological Sciences and Biotechnology, State Key Laboratory of Biomembrane and Membrane Biotechnology, Tsinghua University, Beijing, 100084, China.
J Mater Sci Mater Med. 2006 Sep;17(9):815-23. doi: 10.1007/s10856-006-9840-3.
Three different porous scaffolds were tested. The first two were prepared by sintering bovine bone. The third scaffold was prepared using three-dimensional gel-lamination, a new rapid prototyping method, and was named as hydroxyapatite artificial bone. X-ray diffraction and Fourier transform infrared spectroscopy analysis confirmed that the samples were mainly highly crystalline hydroxyapatite ceramics. Scanning electron microscopy and mercury intrusion porosimetry measurement showed that the pores were interconnected and pore sizes ranged from several microns to hundreds of microns. Mouse osteoblast-like cells grown on the three scaffolds retained their characteristic morphology. Cell proliferation and differentiation, analyzed by methylthiazol tetrazolium (MTT) and alkaline phosphatase activity assays, were significantly higher on the hydroxyapatite artificial bone than on the other two scaffolds tested. All the scaffolds provided good attachment, proliferation and differentiation of bone cells. These results indicate that the scaffolds have a favorable interaction with cells, they support cell growth and functions, and therefore these scaffolds may have great potential as bone substitutes. The three-dimensional gel-lamination method is proven to be an attractive process to design and fabricate bone scaffolds with favorable properties, and therefore, has promising potential for bone repair applications.
对三种不同的多孔支架进行了测试。前两种是通过烧结牛骨制备的。第三种支架采用三维凝胶层压法制备，这是一种新型快速成型方法，被命名为羟基磷灰石人工骨。X射线衍射和傅里叶变换红外光谱分析证实，样品主要是高度结晶的羟基磷灰石陶瓷。扫描电子显微镜和压汞孔隙率测定表明，孔隙相互连通，孔径范围从几微米到几百微米。在这三种支架上生长的小鼠成骨样细胞保持了其特征形态。通过甲基噻唑四氮唑（MTT）和碱性磷酸酶活性测定分析，羟基磷灰石人工骨上的细胞增殖和分化明显高于另外两种测试支架。所有支架都能使骨细胞良好附着、增殖和分化。这些结果表明，这些支架与细胞具有良好的相互作用，它们支持细胞生长和功能，因此这些支架作为骨替代物可能具有巨大潜力。三维凝胶层压法被证明是一种设计和制造具有良好性能的骨支架的有吸引力的工艺，因此在骨修复应用方面具有广阔的前景。
