用于直接组织附着的溶胶-凝胶法制备的TiO2-SiO2植入涂层。第二部分：细胞反应评估。
Sol-Gel-derived TiO2-SiO2 implant coatings for direct tissue attachment. Part II: Evaluation of cell response.
作者信息
Areva Sami, Aäritalo Virpi, Tuusa Sari, Jokinen Mika, Lindén Mika, Peltola Timo
机构信息
Department of Physical Chemistry, Abo Akademi University, Porthansgatan 3-5, 20500 Turku, Finland.
出版信息
J Mater Sci Mater Med. 2007 Aug;18(8):1633-42. doi: 10.1007/s10856-007-3064-z.
Silica-releasing sol-gel derived TiO2-SiO2 coatings with tailored nanostructure were evaluated in fibroblast and osteoblast cell cultures. The adhesion of both fibroblasts and osteoblasts proceeded within two hours. The highest fibroblast proliferation activities were observed on the TiO2-SiO2 (70:30) and (30:70) coatings. However, the cell layer on TiO2-SiO2 (30:70) coating was disordered. Prolonged osteoblast activity was observed on the coatings as a function of increased amount of released silica. At day 21 the surfaces were fully covered by the calcified nodules and extracellular matrix except for the coatings TiO2-SiO2 (10:90) i.e. having the highest SiO2 amount. The results suggested that TiO2-SiO2 (70:30) was the best for fibroblasts and TiO2-SiO2 (30:70) for osteoblasts. The applicability of the sol-gel derived TiO2 and TiO2-SiO2 coatings as an alternative for the calcium phosphate based implant coatings are discussed.
对具有定制纳米结构的二氧化钛-二氧化硅溶胶-凝胶涂层在成纤维细胞和成骨细胞培养中进行了评估。成纤维细胞和成骨细胞的黏附在两小时内完成。在二氧化钛-二氧化硅（70:30）和（30:70）涂层上观察到最高的成纤维细胞增殖活性。然而，二氧化钛-二氧化硅（30:70）涂层上的细胞层是无序的。随着释放的二氧化硅量增加，在涂层上观察到成骨细胞活性延长。在第21天，除了二氧化钛-二氧化硅（10:90）涂层（即二氧化硅含量最高的涂层）外，表面完全被钙化结节和细胞外基质覆盖。结果表明，二氧化钛-二氧化硅（70:30）对成纤维细胞最好，二氧化钛-二氧化硅（30:70）对成骨细胞最好。讨论了溶胶-凝胶衍生的二氧化钛和二氧化钛-二氧化硅涂层作为磷酸钙基植入物涂层替代品的适用性。
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