Ershova L I, Zeĭnalov A M, Azhigirova M A, Dereza T L, Kurbanova G N, Gorbunova N A
Biull Eksp Biol Med. 1992 Feb;113(2):122-4.
The kidney-dependent increase of the haemolytic activity of blood serum after acute decompensated blood loss was demonstrated in the experiments on rats. The preparative ion-exchange chromatography on DEAE-Toyoperal was used to separate the haemolytic active component of the posthaemorrhagic blood serum with the max value of the specific activity of 6.86 A/micrograms protein. The analysis of the separated component by size-exclusion chromatography on TSK-G3000-SW column indicated a molecular mass of 80-100 kDa. In injection in the circulatory system in in vivo experiments the dose-dependent effect of the action of separated component was demonstrated.
在对大鼠的实验中证实，急性失代偿性失血后血清溶血活性的增加依赖于肾脏。使用DEAE - Toyoperal进行制备性离子交换色谱法，以分离出血清中溶血活性成分，其比活性最大值为6.86 A/微克蛋白质。在TSK - G3000 - SW柱上通过尺寸排阻色谱法对分离出的成分进行分析，表明其分子量为80 - 100 kDa。在体内实验中向循环系统注射时，证实了分离成分作用的剂量依赖性效应。
