Schmitt B, Henderson L
National Veterinary Services Laboratories, 1800 Dayton Avenue, Ames Iowa 50010, USA.
Rev Sci Tech. 2005 Apr;24(1):243-50.
While traditional testing methods are still widely used in veterinary diagnostic laboratories exciting new technologies, such as biosensors and microarray techniques, are being developed. Nucleic acid diagnostic techniques such as polymerase chain reaction (PCR) have become routine diagnostic tools in veterinary laboratories not only to make specific typing determinations but also to rapidly screen large numbers of samples during disease outbreaks. In addition, nanotechnologies, although not yet implemented in veterinary laboratories, hold the promise of screening for numerous pathogens in a single assay. Other biotechnologies are likely to be widely used in the future as they can improve diagnostic capabilities while reducing the time and perhaps, the costs, associated with conventional technologies. This paper describes some of these new technologies and concludes that although a lot of developmental work is still required, biotechnology and its applications hold great promise for improving the speed and accuracy of diagnostics for veterinary pathogens.
虽然传统检测方法仍在兽医诊断实验室中广泛使用,但诸如生物传感器和微阵列技术等令人兴奋的新技术正在不断发展。核酸诊断技术,如聚合酶链反应(PCR),已成为兽医实验室的常规诊断工具,不仅用于进行特定的分型测定,还用于在疾病爆发期间快速筛查大量样本。此外,纳米技术虽然尚未在兽医实验室中得到应用,但有望在一次检测中筛查多种病原体。其他生物技术未来可能会被广泛使用,因为它们可以提高诊断能力,同时减少与传统技术相关的时间和成本。本文介绍了其中一些新技术,并得出结论,尽管仍需要大量的研发工作,但生物技术及其应用在提高兽医病原体诊断的速度和准确性方面具有巨大潜力。