Ashraf Aqeela, Imran Muhammad
Institute of Biochemistry and Biotechnology, University of Veterinary and Animal Sciences, Lahore, 54000, Pakistan.
Trop Anim Health Prod. 2018 Aug;50(6):1193-1202. doi: 10.1007/s11250-018-1629-0. Epub 2018 Jun 8.
Accurate diagnosis of disease is the major step between the cause and cure of disease. An economical, reliable, and rapid diagnostic tool is fundamental for the management of udder health. The earlier the disease is identified, the less will be the damage; keeping this in mind, many efforts are being made to develop reliable diagnostic tools for use on farm. However, traditional gold standard methods including somatic cell count and microbial culturing are still in use. They are partially being replaced with polymerase chain reaction and sequencing-based tests. Nanotechnology and protein-based tests have also gained lot of attention and some of them are potential candidate of future diagnostic tests for bovine mastitis. Research laboratories are struggling to develop simple, economical, and user-friendly biosensor-based methods that can be performed on farm for rapid diagnosis. The combination of both genomic and proteomic approaches, together with further involvement of nanotheranostic technologies and other sensors, will assist in the quest of better mastitis diagnostic tools.
疾病的准确诊断是病因与治愈之间的主要环节。一种经济、可靠且快速的诊断工具对于乳房健康管理至关重要。疾病发现得越早，损害就越小；鉴于此，人们正在努力开发适用于农场的可靠诊断工具。然而，包括体细胞计数和微生物培养在内的传统金标准方法仍在使用。它们正部分地被聚合酶链反应和基于测序的检测所取代。纳米技术和基于蛋白质的检测也备受关注，其中一些是未来牛乳腺炎诊断检测的潜在候选方法。研究实验室正在努力开发简单、经济且用户友好的基于生物传感器的方法，以便在农场进行快速诊断。基因组学和蛋白质组学方法的结合，再加上纳米诊疗技术和其他传感器的进一步参与，将有助于寻求更好的乳腺炎诊断工具。
