Greve T, Callesen H
Royal Veterinary and Agricultural University, Department of Large Animal Sciences, Dyrlaegevej 68, 1870 Frederiksberg C, Denmark.
Rev Sci Tech. 2005 Apr;24(1):405-12.
During the past thirty years, basic and experimental studies on classical (superovulation; non-surgical recovery and transfer of cattle embryos) and advanced embryo technologies (in vitro embryo production; cloning by somatic cell nuclear transfer) have generated structural and functional information on oocyte development and quality, fertilisation and conceptus development. This information has provided new insight, not only into these technologies per se but also into the factors contributing to fertility in cattle. It is now known that the peripheral and follicular endocrine profiles have a profound influence on the subsequent developmental competence of the embryo. It is also well established that manipulation of the oocytes or embryos may adversely affect embryonic and foetal development, leading to the so-called 'large offspring syndrome'. Information from such studies has alerted scientists to the importance of epigenetics in cattle reproduction.
在过去三十年中，关于经典（超数排卵；牛胚胎的非手术回收与移植）和先进胚胎技术（体外胚胎生产；体细胞核移植克隆）的基础研究和实验研究，已经产生了关于卵母细胞发育与质量、受精以及胚胎发育的结构和功能信息。这些信息不仅为这些技术本身提供了新的见解，也为影响牛繁殖力的因素提供了新的认识。现在已知外周和卵泡内分泌谱对胚胎随后的发育能力有深远影响。同样确定的是，对卵母细胞或胚胎的操作可能会对胚胎和胎儿发育产生不利影响，导致所谓的“大后代综合征”。这些研究的信息使科学家们意识到表观遗传学在牛繁殖中的重要性。
