维持纤维素酯膜的离子渗透性。

Maintaining the ionic permeability of a cellulose ester membrane.

作者信息

Runge Steven W, Shelton Kyla R, Melton Shane A, Moran William M

机构信息

Department of Biology, 180 Lewis Science Center, University of Central Arkansas, Conway, AR 72035-5003, USA.

出版信息

J Biochem Biophys Methods. 2005 Sep 30;64(3):200-6. doi: 10.1016/j.jbbm.2005.07.003.

DOI:10.1016/j.jbbm.2005.07.003

PMID:16112200

Abstract

This paper reports the results of a series of experiments designed to test conditions that would permit NaCl to diffuse through 100 Da molecular weight cut-off (MWCO) and 1,000 Da MWCO membranes. For the 100 Da MWCO membrane, the membrane becomes completely impermeable to NaCl when dialyzed against distilled water (DW), but inclusion of one of a variety of different salts in the dialyzing solution maintains membrane permeability to NaCl. A titration experiment revealed that a minimum concentration of 0.1 mM of a salt such as KH2PO4 is required to sustain membrane permeability. In contrast, diffusion through the 1,000 Da MWCO membrane was slightly higher when DW was used as the dialysate. We conclude that the 100 Da MWCO membrane works well for a variety of dialysis applications provided that a maintenance salt is included in all dialyzing solutions.

摘要

本文报告了一系列实验的结果，这些实验旨在测试能使氯化钠扩散通过截留分子量为100道尔顿（MWCO）和1000道尔顿MWCO的膜的条件。对于截留分子量为100道尔顿的膜，当用蒸馏水（DW）透析时，该膜对氯化钠变得完全不可渗透，但在透析液中加入各种不同盐中的一种可保持膜对氯化钠的通透性。一项滴定实验表明，维持膜通透性所需的盐（如磷酸二氢钾）的最低浓度为0.1 mM。相比之下，当使用DW作为透析液时，通过截留分子量为1000道尔顿的膜的扩散略高。我们得出结论，只要在所有透析液中都包含维持盐，截留分子量为100道尔顿的膜适用于各种透析应用。