Galindo María, Santiago Begoña, Palao Guillermo, Gutierrez-Cañas Irene, Ramirez Juan Carlos, Pablos José Luis
Servicio de Reumatología y Unidad de Investigación, Hospital 12 de Octubre, Carretera de Andalucía Km 5.4, 28041 Madrid, Spain.
Peptides. 2005 Sep;26(9):1647-53. doi: 10.1016/j.peptides.2005.02.024.
Angiotensin II (AngII) is considered as a cytokine-like factor displaying a variety of proinflammatory and profibrotic cellular effects. Most of these effects seem mediated by AT1 signaling, whereas AT2 expression and function in adult human cells remain unclear. We have studied AT1 and AT2 expression in different human adult fibroblasts types and analyze their response to AngII. AngII did not induce thymidine incorporation, apoptosis nor collagen gene or protein expression in human fibroblasts. Specific AT1 or AT2 inhibitors did not modify this apparent resistance to AngII. We found abundant expression of both AT1 and AT2 receptors in all human fibroblasts studied, whereas vascular smooth muscle cells (VSMC) which only expressed AT1 receptor, displayed a clear AT1-dependent proliferative response to AngII. These data demonstrate that cultured human adult fibroblasts express both AT1 and AT2 receptor types and this phenomenon is associated with a lack of growth or collagen synthesis responses to AngII.
血管紧张素II（AngII）被认为是一种细胞因子样因子，具有多种促炎和促纤维化的细胞效应。这些效应大多似乎是由AT1信号介导的，而AT2在成人细胞中的表达和功能仍不清楚。我们研究了不同类型成人人类成纤维细胞中AT1和AT2的表达，并分析了它们对AngII的反应。AngII不会诱导人类成纤维细胞中的胸苷掺入、凋亡，也不会诱导胶原蛋白基因或蛋白质表达。特异性AT1或AT2抑制剂不会改变这种对AngII的明显抗性。我们发现在所有研究的人类成纤维细胞中，AT1和AT2受体均有丰富表达，而仅表达AT1受体的血管平滑肌细胞（VSMC）对AngII表现出明显的AT1依赖性增殖反应。这些数据表明，培养的成人人类成纤维细胞同时表达AT1和AT2受体类型，并且这种现象与对AngII缺乏生长或胶原蛋白合成反应有关。
