Rose Amy, Xu Yue, Chen Zuxiong, Fan Zengbin, Stamey Thomas A, McNeal John E, Caldwell Mitchell, Peehl Donna M
Department of Urology, Stanford University School of Medicine, Stanford, CA 94305-5118, USA.
Cancer Lett. 2005 Sep 28;227(2):213-22. doi: 10.1016/j.canlet.2005.01.037.
Primary cultures are widely used to investigate the disease-specific biology of prostate cancer and benign prostatic hyperplasia (BPH). To identify genes differentially expressed between epithelial cells cultured from adenocarcinomas versus BPH tissues, we used probe array technology. Gene expression profiles were evaluated on Affymetrix Human Cancer G110 Array Chips containing approximately 1900 cancer-related genes. After defined statistical analysis, genes that were over-expressed in cancer cultures were identified. Protein expression of four of the differentially expressed genes was measured in immunoblots, and the expression of two other genes was measured by real-time reverse transcription-polymerase chain reaction (RT-PCR). While no gene or protein was consistently over-expressed in all cancer versus BPH cell cultures, cytokeratin 16 protein was highly elevated in several of the cancer cultures, suggesting that a hyperproliferative phenotype may be characteristic of prostate cancer cells.
原代培养物被广泛用于研究前列腺癌和良性前列腺增生（BPH）的疾病特异性生物学特性。为了鉴定从腺癌与BPH组织培养的上皮细胞之间差异表达的基因，我们使用了探针阵列技术。在含有约1900个癌症相关基因的Affymetrix人类癌症G110阵列芯片上评估基因表达谱。经过特定的统计分析后，鉴定出在癌症培养物中过度表达的基因。在免疫印迹中测量了四个差异表达基因的蛋白质表达，并通过实时逆转录-聚合酶链反应（RT-PCR）测量了另外两个基因的表达。虽然在所有癌症与BPH细胞培养物中没有基因或蛋白质持续过度表达，但细胞角蛋白16蛋白在几种癌症培养物中高度升高，表明增殖过度表型可能是前列腺癌细胞的特征。
