Larsson J I Ronny
Department of Cell and Organism Biology, University of Lund, Helgonavagen 3, S-223 62 Lund, Sweden.
J Invertebr Pathol. 2005 Sep;90(1):47-50. doi: 10.1016/j.jip.2005.06.016. Epub 2005 Aug 19.
Fresh and frozen spores of the microsporidia Nosema apis and Nosema bombi were fixed using various fixatives at different times and temperatures. Paraformaldehyde and technical formaldehyde gave results comparable to or better than glutaraldehyde. Increased fixation temperature improved the fixation of spores from terrestrial hosts. Freezing did not destroy the cytology of the spore.
