Hamamura M, Nunez D J, Leng G, Emson P C, Kiyama H
Department of Neuroendocrinology, AFRC Institute of Animal Physiology and Genetics Research, Babraham, U.K.
Brain Res. 1992 Feb 14;572(1-2):42-51. doi: 10.1016/0006-8993(92)90448-i.
Conscious rats were given an intraperitoneal (i.p.) injection of isotonic or hypertonic saline, and killed 10-240 min later. In the hypothalamus of hypertonic saline-injected rats, c-fos-mRNA positive cells were mainly restricted to the supraoptic and paraventricular nuclei and to structures associated with the lamina terminalis of the third ventricle, including in particular the subfornical organ, the organum vasculosum of the lamina terminalis (OVLT) and the median preoptic nucleus. These structures comprise the proposed anterior hypothalamic 'osmoreceptor complex' for regulation of vasopressin release. The time course of the appearance and disappearance of c-fos mRNA signals was similar in all regions. Thus c-fos protein (Fos) may be a common transcription factor in the hypothalamic neural circuits involved in osmoregulation.
给清醒大鼠腹腔注射等渗或高渗盐水，10 - 240分钟后处死。在注射高渗盐水的大鼠下丘脑，c-fos-mRNA阳性细胞主要局限于视上核和室旁核以及与第三脑室终板相关的结构，尤其包括穹窿下器官、终板血管器（OVLT）和视前正中核。这些结构构成了提议的用于调节血管加压素释放的下丘脑前部“渗透压感受器复合体”。c-fos mRNA信号出现和消失的时间进程在所有区域相似。因此，c-fos蛋白（Fos）可能是参与渗透压调节的下丘脑神经回路中的一种常见转录因子。
