Gao Changjun, Xu Lixian, Chai Wei, Sun Xude, Zhang Hui, Zhang Guihe
Department of Anesthesiology, Stomatology Hospital, Fourth Military Medical University, Xi' an, Shaanxi Province, China.
J Surg Res. 2005 Dec;129(2):298-305. doi: 10.1016/j.jss.2005.06.022. Epub 2005 Aug 22.
The aim of this study was to investigate the effects of intraluminal hyperoxygenated solution (HOS) on enterocyte mitochondrial structure and respiratory function after intestinal ischemia-reperfusion (IR) in rabbits.
Thirty-two rabbits were divided randomly into four groups: control group in which sham operation was performed (Sham group), ischemia-reperfusion group (IR group), and two HOS treatment groups (H1 group and H2 group). Intestinal IR model was produced by clamping superior mesenteric artery (SMA) with an atraumatic vascular clamp for 1 h, followed by reperfusion for 2 h. Animals in the H1 group and H2 group received intraluminal HOS infusion for 1 h immediately after occlusion of SMA, and the rates of infusion were 10 and 20 mL/kg.h, respectively. After 2 h of reperfusion, enterocyte mitochondria morphological quantitative analysis was made with electron microscopy and biogenetics stereology, and the following parameters, including mitochondrial respiratory control ratio (RCR), intestinal O(2) extraction ratio (ER), and mucosal ATP contents, were measured, respectively.
After IR, the mitochondria was severely swollen with broken cristae, and mean transaction area, diameter, surface density, and volume density of the mitochondria increased significantly. Meanwhile, specific surface and numeral density of the mitochondria decreased significantly. The mitochondrial RCR, intestinal O(2) ER, and mucosal ATP contents were all decreased significantly. There were no differences in all parameters between the IR group and H1 group. In the H2 group, the mitochondria were slightly swollen, and mean transaction area, diameter, surface density, and volume density of the mitochondria were all significantly lower, with the specific surface and numeral density of the mitochondria significantly higher compared with the IR group. The mitochondrial RCR, intestinal O(2) ER and mucosal ATP contents in H2 group were all significantly higher than those in IR group.
Intraluminal HOS infusion at 20 mL/kg.h during ischemia ameliorates structural and functional changes of enterocyte mitochondria associated with intestinal IR injury, which is a safe, simple, and effective measure to protect the intestine from IR injury.
本研究旨在探讨管腔内高氧溶液（HOS）对兔肠缺血再灌注（IR）后肠上皮细胞线粒体结构及呼吸功能的影响。
32只兔随机分为四组：行假手术的对照组（假手术组）、缺血再灌注组（IR组）和两个HOS治疗组（H1组和H2组）。采用无损伤血管夹夹闭肠系膜上动脉（SMA）1小时制备肠IR模型，随后再灌注2小时。H1组和H2组动物在夹闭SMA后立即管腔内输注HOS 1小时，输注速率分别为10和20 mL/kg·h。再灌注2小时后，用电子显微镜和生物遗传学体视学对肠上皮细胞线粒体进行形态定量分析，并分别测定线粒体呼吸控制率（RCR）、肠氧摄取率（ER）和黏膜ATP含量等参数。
IR后，线粒体严重肿胀，嵴断裂，线粒体的平均横截面积、直径、表面密度和体积密度显著增加。同时，线粒体的比表面积和数量密度显著降低。线粒体RCR、肠氧ER和黏膜ATP含量均显著降低。IR组和H1组所有参数均无差异。H2组线粒体轻度肿胀，线粒体的平均横截面积、直径、表面密度和体积密度均显著低于IR组，线粒体的比表面积和数量密度显著高于IR组。H2组线粒体RCR、肠氧ER和黏膜ATP含量均显著高于IR组。
缺血期间以20 mL/kg·h的速率管腔内输注HOS可改善与肠IR损伤相关的肠上皮细胞线粒体结构和功能变化，这是一种安全、简单且有效的保护肠道免受IR损伤的措施。
