超氧液在一氧化碳中毒治疗策略中的潜在应用。

Potential use of hyperoxygenated solution as a treatment strategy for carbon monoxide poisoning.

机构信息

Department of Anesthesiology, School of Stomatology, the Fourth Military Medical University, Xi'an, China.

出版信息

PLoS One. 2013 Dec 2;8(12):e81779. doi: 10.1371/journal.pone.0081779. eCollection 2013.

DOI:10.1371/journal.pone.0081779

PMID:24312588

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3847038/

Abstract

AIM

Carbon monoxide (CO) poisoning can cause permanent damage in tissues that are sensitive to hypoxia. We explored the feasibility and efficacy of using a hyperoxygenated solution (HOS) to treat severe acute CO poisoning in an animal model.

METHODS

Male Sprague-Dawley rats were subjected to CO poisoning. The HOS was administered into the femoral vein of these rats through a catheter (10 ml/kg). Carboxyhemoglobin (COHb) and blood gases were used to assess the early damage caused by CO poisoning. S100β was measured to predict the development of late cognitive sequelae of CO. The Morris water maze test was performed to assess cognitive function, and Nissl staining was performed to observe histologic change.

RESULTS

The COHb concentrations rapidly decreased at 5 min after the HOS administration; however, the PaO2 and SaO2 in rats treated with HOS increased significantly 5 min after the HOS administration. The S100β concentrations, which increased significantly after CO poisoning, increased at a much slower rate in the rats treated with HOS (HOS group) compared with the rats treated with O2 inhalation (O2 group). The escape latency in the place navigation test was shortened after CO poisoning on days 11-15 and days 26-30, and the swimming time in quadrant 4 in the spatial probe test on days 15 and 30 after CO poisoning was prolonged in the rats treated with HOS injection compared with the rats treated with oxygen inhalation or normal saline injection. The neuronal degeneration in the HOS group was alleviated than that in the CO or O2 group.

CONCLUSION

HOS efficiently alleviates the brain damage in acute CO-poisoned rats and thus may serve as a new way to treat human patients with CO poisoning in clinical practice.

摘要

目的

一氧化碳（CO）中毒会导致对缺氧敏感的组织永久性损伤。我们在动物模型中探索了使用高氧溶液（HOS）治疗重度急性 CO 中毒的可行性和疗效。

方法

雄性 Sprague-Dawley 大鼠进行 CO 中毒。通过导管将 HOS （10 ml/kg）注入这些大鼠的股静脉。用碳氧血红蛋白（COHb）和血气来评估 CO 中毒引起的早期损伤。用 S100β来预测 CO 迟发性认知后遗症的发展。通过 Morris 水迷宫测试评估认知功能，并用尼氏染色观察组织学变化。

结果

HOS 给药后 5 分钟，COHb 浓度迅速下降；然而，HOS 治疗组大鼠的 PaO2 和 SaO2 在 HOS 给药后 5 分钟显著增加。CO 中毒后明显升高的 S100β浓度在 HOS 治疗组（HOS 组）中升高速度明显较慢。CO 中毒后第 11-15 天和第 26-30 天的位置导航试验中，逃避潜伏期缩短，CO 中毒后第 15 天和第 30 天空间探测试验中象限 4 的游泳时间延长。与吸氧或生理盐水治疗相比，HOS 治疗组大鼠的神经元变性减轻。