Namkinga L A, Matee M I N, Kivaisi A K, Kullaya A, Mneney E E
Applied Microbiology Unit, Department of Botany, Faculty of Medicine, Muhimbili University College of Health Sciences, Dar es Salaam, Tanzania.
East Afr Med J. 2005 May;82(5):226-34. doi: 10.4314/eamj.v82i5.9311.
To identify Candida strains isolated from Tanzanian women (13 to 45 years) with vaginal candidiasis.
A cross-sectional study.
Antenatal clinic in Ilala district hospital in Dar es Salaam, Tanzania from March 1998 to December 2000.
The identities of the 272 isolates tested with API Candida were: Candida albicans 180(66.2%), Candida tropicalis 13(4.7%), Candida glabrata 20(7.35%), Candida famata 6(2.2%), Candida parapsilosis 6 (2.2%) and Candida lusitaniae one (0.37%). API Candida could not speciate 43 (15.8%) isolates of these; two (0.7%) fell between C. albicans and C. tropicalis, 17(6.25%) C. lusitaniael, C. guilliermondii/C. famata, 14(5.15%) C. krusei, C. inconspicua, and C. norvegensis and nine (3.3%) either C. parapsilosis, C. krusei, C. incospicua or Geotrichum spp. Four (1.5%) isolates had an assimilation pattern of Trichosporo spp, but were all germ tube positive and had morphological features on cornemeal agar that were consistent with C. albicans. API 20C AUX was used for testing 29 isolates and results showed: 11/29 (37.9%) C. albicans, 1/29 (3.4%) C. tropicalis, 4/29 (13.8%) C. glabrata, 1/29 (3.4%) C. parapsilosis, 1/29 (3.4%) C. famata, 1/29 (3.4%) C. lusitaniae, 1/29 (3.4%) C. colliculosa/C. magnoliae, 5/29(17.2%) C. albicans/ C. tropicals 2/29 (6.8%) C. norvegensis/C. parapsilosis, and 2/29(6.8%) C. kruseil/C. inconspicua. Results of 20 isolates identified by Randomly Amplified Polymorphic DNA (RAPID) technique showed a 95% agreement with API Candida and a 100% agreement with API 20C AUX.
Although most (66.3%) of the species isolated from Dar es Salaam women with vaginal candidiasis were C. albicans, a considerable percentage (33.7%) were non-albicans, mainly C. glabrata, C. krusei and C. tropicalis. The high prevalence of non-albicans Candida spp observed in this population may have therapeutic implications.
鉴定从坦桑尼亚患有阴道念珠菌病的女性（13至45岁）中分离出的念珠菌菌株。
一项横断面研究。
1998年3月至2000年12月在坦桑尼亚达累斯萨拉姆伊拉拉区医院的产前诊所。
用API念珠菌测试的272株分离株的鉴定结果为：白色念珠菌180株（66.2%），热带念珠菌13株（4.7%），光滑念珠菌20株（7.35%），法塔念珠菌6株（2.2%），近平滑念珠菌6株（2.2%），葡萄牙念珠菌1株（0.37%）。API念珠菌无法鉴定其中43株（15.8%）分离株；2株（0.7%）介于白色念珠菌和热带念珠菌之间，17株（6.25%）介于葡萄牙念珠菌、季也蒙念珠菌/法塔念珠菌之间，14株（5.15%）介于克鲁斯念珠菌、隐匿念珠菌和挪威念珠菌之间，9株（3.3%）介于近平滑念珠菌、克鲁斯念珠菌、隐匿念珠菌或地丝菌属之间。4株（1.5%）分离株具有毛孢子菌属的同化模式，但均芽管阳性，在玉米粉琼脂上的形态特征与白色念珠菌一致。使用API 20C AUX对29株分离株进行测试，结果显示：白色念珠菌11/29（37.9%），热带念珠菌1/29（3.4%），光滑念珠菌4/29（13.8%），近平滑念珠菌1/29（3.4%），法塔念珠菌1/29（3.4%），葡萄牙念珠菌1/29（3.4%），颈圈念珠菌/木兰念珠菌1/29（3.4%），白色念珠菌/热带念珠菌5/29（17.2%），挪威念珠菌/近平滑念珠菌2/29（6.8%），克鲁斯念珠菌/隐匿念珠菌2/29（6.8%）。通过随机扩增多态性DNA（RAPD）技术鉴定的20株分离株的结果与API念珠菌的一致性为95%，与API 20C AUX的一致性为100%。
尽管从达累斯萨拉姆患有阴道念珠菌病的女性中分离出的大多数（66.3%）菌株是白色念珠菌，但相当比例（33.7%）是非白色念珠菌，主要是光滑念珠菌、克鲁斯念珠菌和热带念珠菌。在该人群中观察到的非白色念珠菌属的高流行率可能具有治疗意义。
