Huang Yun, Yang Huan-Jie, Jin Yan, Li Hui-Min, Fu Song-Bin
Laboratory of Medical Genetics, Harbin Medical University, Harbin 150086, China.
Yi Chuan. 2005 Jul;27(4):531-4.
A large number of numerical and structural aberrations were analyzed in human tumor metastatic cells and 13q14 aberrations were frequently detected in some types of metastatic cancers. The rearrangement of 13q14 was identified previously in two lung adenocarcinoma cell lines with the same origin but different metastatic potential AGZY83-a and Anip973. BRI gene showed different expression levels in the cell lines as revealed by mRNA differential display (mRNA DD) in the two cell lines, and located in 13q14. In order to investigate the relationship between 13q14 abnormalities and tumor metastasis, a painting probe (13q) was used to hybridize three G-banded NSCLC cell lines with different metastatic potential. The major abnormality of 13q differs among different cell lines, including 13q32-33 frequent breakpoint in these three cell lines. But low metastatic potential cell lines PAa, SPC-1-A were not found breakpoint in 13q14, while 95D cell line with high metastatic potential had the common breakpoint 13q14 in two cell clones. The results suggested that the breakage at 13q14 may possibly be related to lung cancer metastasis. The affirmative relationship between 13q14 aberration and NSCLC needs further investigation.
对人肿瘤转移细胞中的大量数值和结构畸变进行了分析，在某些类型的转移性癌症中经常检测到13q14畸变。先前在两个起源相同但转移潜能不同的肺腺癌细胞系AGZY83-a和Anip973中鉴定出13q14的重排。通过两个细胞系中的mRNA差异显示（mRNA DD）发现，BRI基因在细胞系中表现出不同的表达水平，且定位于13q14。为了研究13q14异常与肿瘤转移之间的关系，使用一个染色体涂染探针（13q）与三个具有不同转移潜能的G显带非小细胞肺癌细胞系进行杂交。13q的主要异常在不同细胞系中有所不同，包括这三个细胞系中常见的13q32 - 33断点。但低转移潜能细胞系PAa、SPC - 1 - A在13q14未发现断点，而高转移潜能的95D细胞系在两个细胞克隆中均有共同的13q14断点。结果提示，13q14的断裂可能与肺癌转移有关。13q14畸变与非小细胞肺癌之间的确切关系需要进一步研究。
