Chen Ying-zhun, Li Yu, Zou Rong, Chen Yu, Wang Yan-ying, Feng Hui-chen, Wang Wu-ru
Department of Pathology, Second Affiliated Hospital of Harbin Medical University, Harbin 150086, China.
Zhonghua Bing Li Xue Za Zhi. 2004 Feb;33(1):62-6.
To investigate the potential relationship between BRI gene expression and metastatic potential in human non-small cell lung cancer (NSCLC).
Using semi-quantitative reverse-transcription polymerase chain reaction (RT-PCR) and Northern blot hybridization techniques, differential expression of the BRI gene in human lung adenocarcinoma cell lines AGZY-83-a and Anip-973 was investigated. Having a much higher metastatic potential, Anip-973 was isolated from AGZY-83-a parental cell line. In addition, the other 6 non-small cell lung cancer cell lines (SPC-A-1, A549, 95D, TKB-18, GLC-82, PAa) and 30 samples of lung cancer tissues with matched corresponding adjacent normal tissues were also analyzed.
There were significant differences in BRI gene expression between the two cell lines. BRI was preferentially expressed in Anip-973 cells compared to its parental cell line AGZY-83-a, and was also up-regulated in the other 6 lung cancer cell lines, correlating possibly with their metastatic potentials. BRI gene over-expression was observed in 30 lung cancer tissues compared with its corresponding adjacent normal tissues. A relative over-expression of BRI mRNA (tumor/normal >or= 2) was observed in 6 of 8 cancer samples with lymph node metastasis and 10 of 22(45.5%) samples without lymph node metastasis. Furthermore, two mRNA transcripts of BRI gene were observed: a 2.0 kb transcript which was mainly observed in normal lung tissues and a 1.6 kb transcript which was present as a dominant species in cancer tissues.
BRI mRNA expression is significantly up-regulated in NSCLC cell lines and clinical tumor samples. An alternatively spliced 1.6 kb mRNA is a major transcript of the gene in NSCLCs, suggesting that differential RNA processing and expression of BRI gene may play a role in the tumorigenesis and/or be related to the metastatic potential of human lung cancer.
研究BRI基因表达与人类非小细胞肺癌（NSCLC）转移潜能之间的潜在关系。
采用半定量逆转录聚合酶链反应（RT-PCR）和Northern印迹杂交技术，研究BRI基因在人肺腺癌细胞系AGZY-83-a和Anip-973中的差异表达。Anip-973具有更高的转移潜能，它是从AGZY-83-a亲代细胞系中分离出来的。此外，还分析了其他6个人非小细胞肺癌细胞系（SPC-A-1、A549、95D、TKB-18、GLC-82、PAa）以及30例肺癌组织及其相应的配对癌旁正常组织样本。
两个细胞系之间BRI基因表达存在显著差异。与亲代细胞系AGZY-83-a相比，BRI在Anip-973细胞中优先表达，并且在其他6个肺癌细胞系中也上调，这可能与其转移潜能相关。与相应的癌旁正常组织相比，在30例肺癌组织中观察到BRI基因过表达。在8例有淋巴结转移的癌样本中有6例以及22例（45.5%）无淋巴结转移的样本中有10例观察到BRI mRNA相对过表达（肿瘤/正常≥2）。此外，观察到BRI基因的两种mRNA转录本：一种2.0 kb的转录本主要在正常肺组织中观察到，另一种1.6 kb的转录本在癌组织中为主要类型。
BRI mRNA表达在NSCLC细胞系和临床肿瘤样本中显著上调。一种选择性剪接的1.6 kb mRNA是该基因在NSCLC中的主要转录本，提示BRI基因的差异RNA加工和表达可能在肿瘤发生中起作用和/或与人类肺癌的转移潜能相关。
