Chen Tian-Feng, Zheng Wen-Jie, Luo Yong, Yang Fang, Bai Yan, Tu Fang
Department of Chemistry, Jinan University, China.
Zhi Wu Sheng Li Yu Fen Zi Sheng Wu Xue Xue Bao. 2005 Aug;31(4):369-73.
Changes in photosynthetic pigment and protein contents, growth and the spectral characteristics in Chlorella vulgaris in response to selenium stress were investigated. Carotenoids (beta-carotene and xanthophylls) and chlorophyll (Chl a and Chl b) contents in cells exposed to Se 50 mg/L increased primarily and decreased afterwards, while photosynthetic pigments in cells exposed to Se 800 mg/L decreased significantly. Chlorophyll (Chl) absorption peak at 693 nm and prominent Chl emission peak at 700 nm, weakened significantly after Se stress. The excitation spectra showed a decrease in excitation energy transfer efficiency in Se-stressed cells. Total soluble protein decreased after Se stress. The changes in total Se, Mg(2+), Ca(2+), K(+) and Na(+) concentrations in culture medium and cells were also determined by ICP-AES.
研究了普通小球藻在硒胁迫下光合色素和蛋白质含量、生长及光谱特征的变化。暴露于50 mg/L硒的细胞中类胡萝卜素（β-胡萝卜素和叶黄素）和叶绿素（叶绿素a和叶绿素b）含量先增加后减少，而暴露于800 mg/L硒的细胞中光合色素显著减少。硒胁迫后，693 nm处的叶绿素吸收峰和700 nm处突出的叶绿素发射峰显著减弱。激发光谱显示硒胁迫细胞中激发能量转移效率降低。硒胁迫后总可溶性蛋白减少。还通过电感耦合等离子体发射光谱法测定了培养基和细胞中总硒、镁离子、钙离子、钾离子和钠离子浓度的变化。
