鸵鸟（鸵鸟属骆驼鸵鸟）胰腺中胰蛋白酶原、胰腺分泌型胰蛋白酶抑制剂以及多种形式的糜蛋白酶原和胰蛋白酶的分离与部分特性研究

The isolation and partial characterization of trypsinogen, pancreatic secretory trypsin inhibitor and multiple forms of chymotrypsinogen and trypsin from the pancreas of the ostrich (Struthio camelus).

Smith N, Naudé R J, Oelofsen W, Lazure C, Patthy A

Department of Biochemistry, University of Port Elizabeth, South Africa.

Int J Biochem. 1992 Jun;24(6):877-85. doi: 10.1016/0020-711x(92)90092-f.

Abstract

PSTI, two chymotrypsinogens and two trypsins were purified to homogeneity by acid extraction, salt fractionation, SP-Sephadex C-50 chromatography and RP-HPLC. 2. A third chymotrypsinogen, a trypsinogen and another trypsin were purified using an alkaline extraction procedure, followed by Trasylol- and Benzamidine-Sepharose affinity chromatography and hydroxylapatite chromatography. 3. The enzymes differed in amino acid composition as well as in specific activities towards synthetic amidase and esterase substrates. 4. N-terminal amino acid sequences were determined for one chymotrypsinogen and one trypsin.

摘要

通过酸提取、盐分级分离、SP-葡聚糖凝胶C-50色谱法和反相高效液相色谱法，将胰腺分泌性胰蛋白酶抑制剂（PSTI）、两种胰凝乳蛋白酶原和两种胰蛋白酶纯化至同质。2. 使用碱性提取程序，随后进行抑肽酶和苯甲脒琼脂糖亲和色谱法以及羟基磷灰石色谱法，纯化出第三种胰凝乳蛋白酶原、一种胰蛋白酶原和另一种胰蛋白酶。3. 这些酶在氨基酸组成以及对合成酰胺酶和酯酶底物的比活性方面存在差异。4. 测定了一种胰凝乳蛋白酶原和一种胰蛋白酶的N端氨基酸序列。