Garí E, Toledo J C, Gibert I, Barbé J
Department of Genetics and Microbiology, Faculty of Sciences, Autonomous University of Barcelona, Spain.
FEMS Microbiol Lett. 1992 May 15;72(1):103-8. doi: 10.1016/0378-1097(92)90497-c.
The nucleotide sequence of the 1794-bp fragment containing the crtD gene from Rhodobacter sphaeroides 2.4.1 encoding for methoxyneurosporene dehydrogenase has been determined. A 63% sequence identity was found when compared with the nucleotide sequence of the crtD gene from Rhodobacter capsulatus. A putative regulatory palindromic motif present in the crtD gene from R. capsulatus also exists in this gene from R. sphaeroides. The translated open reading frame of the crtD gene of R. sphaeroides has identified a polypeptide of 495 amino acids which shares a 56% sequence identity with the same CrtD protein of R. capsulatus. The N- and C-termini of these CrtD proteins present a high degree of similarity with the N- and C-termini of other carotenoid dehydrogenases including those encoded by crtI genes. This is in good agreement with the previously hypothesized homology between CrtI and CrtD proteins.
已确定来自球形红杆菌2.4.1的编码甲氧基神经孢烯脱氢酶的1794 bp片段的核苷酸序列，该片段包含crtD基因。与来自荚膜红杆菌的crtD基因的核苷酸序列相比，发现有63%的序列同一性。荚膜红杆菌crtD基因中存在的一个假定的调控回文基序在球形红杆菌的该基因中也存在。球形红杆菌crtD基因的翻译开放阅读框已鉴定出一个由495个氨基酸组成的多肽，该多肽与荚膜红杆菌的相同CrtD蛋白具有56%的序列同一性。这些CrtD蛋白的N端和C端与其他类胡萝卜素脱氢酶的N端和C端高度相似，包括那些由crtI基因编码的脱氢酶。这与之前假设的CrtI和CrtD蛋白之间的同源性高度一致。
