Park J C, Bai S, Tai C Y, Chun S B
Department of Microbiology, College of Natural Sciences, Chonnam National University, Kwang-ju, Korea.
FEMS Microbiol Lett. 1992 May 15;72(1):17-23. doi: 10.1016/0378-1097(92)90483-5.
The Schwanniomyces occidentalis (formerly castellii) ATCC 26077 (CBS 2863) alpha-amylase (AMY 26077) gene was cloned in Saccharomyces cerevisiae and sequenced. An open-reading frame encoding the AMY consists of 1536 base pairs and contains 512 amino-acid residues, which is almost the same in size as the AMY of Sch. occidentalis ATCC 26076 and CCRC 21164. The amino-acid sequence of AMY 26077 differed from that of ATCC 26076 alpha-amylase (AMY 26076) at two residues and from that of CCRC 21164 alpha-amylase (AMY 21164) at three residues. Comparison of the AMY 26077 gene with its homologues from two other strains (Sch. alluvius CBS 1153 and Sch. persoonii CBS 2169) using several restriction enzymes revealed that the AMY 26077 was very similar to AMY CBS 1153 but different from that of CBS 2169.
西方施旺酵母（原卡氏酵母）ATCC 26077（CBS 2863）的α-淀粉酶（AMY 26077）基因在酿酒酵母中克隆并测序。编码AMY的开放阅读框由1536个碱基对组成，包含512个氨基酸残基，其大小与西方施旺酵母ATCC 26076和CCRC 21164的AMY几乎相同。AMY 26077的氨基酸序列与ATCC 26076α-淀粉酶（AMY 26076）在两个残基处不同，与CCRC 21164α-淀粉酶（AMY 21164）在三个残基处不同。使用几种限制酶将AMY 26077基因与其来自其他两个菌株（冲积施旺酵母CBS 1153和佩氏施旺酵母CBS 2169）的同源物进行比较，结果显示AMY 26077与AMY CBS 1153非常相似，但与CBS 2169的不同。
