Abarca D, Fernández-Lobato M, del Pozo L, Jiménez A
Centro de Biologia Molecular (CSIC-UAM), Universidad Autónoma, Spain.
FEBS Lett. 1991 Feb 11;279(1):41-4. doi: 10.1016/0014-5793(91)80245-x.
A new gene (SW A2) encoding a secretory alpha-amylase activity from Schwanniomyces occidentalis has been cloned from this yeast and then expressed in Saccharomyces cerevisiae. Both Sw, occidentalis and a transformant of S. cerevisiae incorporating SW A2 contain a transcript of 2.1 kb which hybridizes to DNa carrying the SW A2 gene. This indicates that the transcript is a product of the SW A2 gene. Transcription of the SW A2 gene seems to be regulated in both Sw. occidentalis and S. cerevisiae. Furthermore, the secretion of alpha-amylase activity is drastically repressed by glucose in both Sw. occidentalis and a transformant of S. cerevisiae containing SW A2.
已从西方许旺酵母中克隆出一个编码分泌性α-淀粉酶活性的新基因（SW A2），并在酿酒酵母中进行了表达。西方许旺酵母和整合了SW A2的酿酒酵母转化体均含有一个2.1 kb的转录本，该转录本与携带SW A2基因的DNA杂交。这表明该转录本是SW A2基因的产物。SW A2基因的转录似乎在西方许旺酵母和酿酒酵母中均受到调控。此外，在西方许旺酵母和含有SW A2的酿酒酵母转化体中，葡萄糖均能强烈抑制α-淀粉酶活性的分泌。
