Mao Li, Jin Nianzu, Chen Jingheng
School of Public Health, Nanjing Medical University, Nanjing 210029, China.
Se Pu. 2005 May;23(3):282-4.
A method for the determination of quercetin in pulp and peel of apples by reversed-phase high performance liquid chromatography with internal standard was developed. Samples were frozen at -80 degrees C for 24 h, then added 6 mol/L hydrochloride under the protection of antioxidant 2,6-di-tert-butyl-p-cresol. The slurry was hydrolyzed thermostatically at 90 degrees C for 2 h and centrifugated for 10 min. The separation was performed on an ODS column (150 mm x 6.0 mm i.d., 5 microm). Methanol-water (55:45, v/v) (pH adjusted to 3.3 with acetic acid) was used as the mobile phase with a flow rate of 1.0 mL/min. The injection volume was 20 microL. The detection wavelength was 370 nm. The results showed that the quercetin content in pulp and in peel were 3.11-10.78 microg/g and 253.57-744.59 microg/g, respectively. The mean recovery of quercetin in apple pulp was 100.4%. The method is simple, accurate, and reliable.
建立了一种采用内标法反相高效液相色谱测定苹果果肉和果皮中槲皮素的方法。样品在-80℃冷冻24小时，然后在抗氧化剂2,6-二叔丁基对甲酚保护下加入6mol/L盐酸。浆液在90℃恒温水解2小时，离心10分钟。分离在ODS柱（150mm×6.0mm内径，5μm）上进行。以甲醇-水（55:45，v/v）（用乙酸调pH至3.3）为流动相，流速为1.0mL/min。进样量为20μL。检测波长为370nm。结果表明，果肉和果皮中槲皮素含量分别为3.11 - 10.78μg/g和253.57 - 744.59μg/g。苹果果肉中槲皮素的平均回收率为100.4%。该方法简便、准确、可靠。
