Liang Taigang, Liu Enli, Zhao Chengxiao, Ban Shurong, Li Qingshan
School of Pharmaceutical Science, Shanxi Medical University, Taiyuan 030001, China.
Zhongguo Zhong Yao Za Zhi. 2009 Sep;34(17):2217-9.
To develop an HPLC method for simultaneous determination of rutin, hyperoside, quercetin and kaempferol in Malus prunifolia from Shanxi province in China.
The separation was performed on a Hypersil C18 column (4.6 mm x 250 mm, 5 microm), using a gradient elution with methanol-water containing 0.2% phosphoric acid as the mobile phase. The flow rate was 1.0 mL x min(-1), the detection wavelength was 360 nm and the temperature of column was 25 degrees T.
The linear ranges of rutin, hyperoside, quercetin and kaempferol were 1.87-46.67 mg x L(-1), 6.40-160.0 mg x L(-1), 3.33-83.33 mg x L(-1), 0.80-20.00 mg x L(-1), respectively. The average recoveries (n=6) of the four constituents were 99.2% (RSD 2.9%), 98.2% (RSD 1.9%), 97.4% (RSD 2.3%), 97.2% (RSD 1.3%), respectively.
The method was simple, accurate and can be used to evaluate medicinal value of Malus prunifolia.
建立一种高效液相色谱法同时测定中国山西省苹果叶中芦丁、金丝桃苷、槲皮素和山奈酚的含量。
采用Hypersil C18柱（4.6 mm×250 mm，5μm）进行分离，以含0.2%磷酸的甲醇-水为流动相进行梯度洗脱。流速为1.0 mL·min⁻¹，检测波长为360 nm，柱温为25℃。
芦丁、金丝桃苷、槲皮素和山奈酚的线性范围分别为1.87 - 46.67 mg·L⁻¹、6.40 - 160.0 mg·L⁻¹、3.33 - 83.33 mg·L⁻¹、0.80 - 20.00 mg·L⁻¹。四种成分的平均回收率（n = 6）分别为99.2%（相对标准偏差2.9%）、98.2%（相对标准偏差1.9%）、97.4%（相对标准偏差2.3%）、97.2%（相对标准偏差1.3%）。
该方法简便、准确，可用于评价苹果叶的药用价值。
