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[反相高效液相色谱法同时测定山西楸子中4种黄酮类化合物]

[Simultaneous determination of four flavonoids in Malus prunifolia from Shanxi province by RP-HPLC].

作者信息

Liang Taigang, Liu Enli, Zhao Chengxiao, Ban Shurong, Li Qingshan

机构信息

School of Pharmaceutical Science, Shanxi Medical University, Taiyuan 030001, China.

出版信息

Zhongguo Zhong Yao Za Zhi. 2009 Sep;34(17):2217-9.

PMID:19943489

Abstract

OBJECTIVE

To develop an HPLC method for simultaneous determination of rutin, hyperoside, quercetin and kaempferol in Malus prunifolia from Shanxi province in China.

METHOD

The separation was performed on a Hypersil C18 column (4.6 mm x 250 mm, 5 microm), using a gradient elution with methanol-water containing 0.2% phosphoric acid as the mobile phase. The flow rate was 1.0 mL x min(-1), the detection wavelength was 360 nm and the temperature of column was 25 degrees T.

RESULT

The linear ranges of rutin, hyperoside, quercetin and kaempferol were 1.87-46.67 mg x L(-1), 6.40-160.0 mg x L(-1), 3.33-83.33 mg x L(-1), 0.80-20.00 mg x L(-1), respectively. The average recoveries (n=6) of the four constituents were 99.2% (RSD 2.9%), 98.2% (RSD 1.9%), 97.4% (RSD 2.3%), 97.2% (RSD 1.3%), respectively.

CONCLUSION

The method was simple, accurate and can be used to evaluate medicinal value of Malus prunifolia.

摘要

目的

建立一种高效液相色谱法同时测定中国山西省苹果叶中芦丁、金丝桃苷、槲皮素和山奈酚的含量。

方法

采用Hypersil C18柱（4.6 mm×250 mm，5μm）进行分离，以含0.2%磷酸的甲醇-水为流动相进行梯度洗脱。流速为1.0 mL·min⁻¹，检测波长为360 nm，柱温为25℃。

结果

芦丁、金丝桃苷、槲皮素和山奈酚的线性范围分别为1.87 - 46.67 mg·L⁻¹、6.40 - 160.0 mg·L⁻¹、3.33 - 83.33 mg·L⁻¹、0.80 - 20.00 mg·L⁻¹。四种成分的平均回收率（n = 6）分别为99.2%（相对标准偏差2.9%）、98.2%（相对标准偏差1.9%）、97.4%（相对标准偏差2.3%）、97.2%（相对标准偏差1.3%）。