Warren Alessandra, Bertolino Patrick, Cogger Victoria C, McLean Allan J, Fraser Robin, Le Couteur David G
Centre for Education and Research on Ageing, University of Sydney, Concord RG Hospital, Sydney, NSW, Australia.
Exp Gerontol. 2005 Oct;40(10):807-12. doi: 10.1016/j.exger.2005.06.012. Epub 2005 Aug 24.
Age-related changes in the hepatic sinusoid of the rat, human and baboons called pseudocapillarization have been discovered and are important because they are considered to be implicated in the pathogenesis of some age-related diseases. In this study, we investigated whether similar changes occur in the livers of old mice. Livers of young (3-4 months) and old (20-24 months) mice were perfusion-fixed and studied using electron microscopy and immunohistochemistry. The thickness of the sinusoidal endothelium was increased in old mice (154+/-4 versus 244+/-8 nm, P<0.001). There was a reduction in fenestrations within the endothelium (porosity decreased from 4.1+/-0.3 to 2.2+/-0.2%, P<0.001). There was perisinusoidal staining with Sirius red in old mice, however, expression of laminin and von Willebrands factor was similar in young and old mice. Novel perisinusoidal fat-engorged stellate cells were found extensively in the old mice. This study confirmed that pseudocapillarization is a widespread aging change in the liver, now documented in several species including the mouse. Mice are an appropriate animal model for studying aging and the hepatic sinusoid.
在大鼠、人类和狒狒的肝血窦中发现了与年龄相关的变化，即所谓的假毛细血管化，这一变化很重要，因为它们被认为与某些年龄相关疾病的发病机制有关。在本研究中，我们调查了老年小鼠肝脏中是否会发生类似变化。对年轻（3 - 4个月）和老年（20 - 24个月）小鼠的肝脏进行灌注固定，并使用电子显微镜和免疫组织化学进行研究。老年小鼠肝血窦内皮厚度增加（154±4对244±8纳米，P<0.001）。内皮细胞窗孔减少（孔隙率从4.1±0.3%降至2.2±0.2%，P<0.001）。老年小鼠肝血窦周围有天狼星红染色，但层粘连蛋白和血管性血友病因子在年轻和老年小鼠中的表达相似。在老年小鼠中广泛发现了新型的肝血窦周围脂肪充盈的星状细胞。本研究证实，假毛细血管化是肝脏中广泛存在的衰老变化，目前已在包括小鼠在内的多个物种中得到记录。小鼠是研究衰老和肝血窦的合适动物模型。
