Cogger Victoria C, Warren Alessandra, Fraser Robin, Ngu Meng, McLean Allan J, Le Couteur David G
Centre for Education and Research on Ageing and ANZAC Research Institute, University of Sydney, Concord RG Hospital, Sydney, NSW 2139, Australia.
Exp Gerontol. 2003 Oct;38(10):1101-7. doi: 10.1016/j.exger.2003.07.002.
BACKGROUND/AIMS: Age-related changes in the hepatic sinusoid termed pseudocapillarization have been reported in the rat and human and have implications for disease susceptibility in old age. In this study, we investigated whether similar changes occur in the livers of old baboons and thus represent a widespread aging change.
Liver tissue from five young baboons (5.4+/-0.5yrs) and five old baboons (21.8+/-0.7yrs) was compared by transmission electron microscopy, scanning electron microscopy and immunohistochemistry.
The thickness of the sinusoidal endothelium was increased in old baboons (130+/-8 nm versus 186+/-9 nm, P<0.001) and the frequency of endothelial fenestrae decreased, with the porosity declining from 4.2+/-0.5% to 2.4+/-0.4% (P=0.006). The expression of laminin and von Willebrands factor was more extensive in old baboons. Novel perisinusoidal ring-shaped cells, probably fat-engorged stellate cells, were prominent in the old baboons.
Pseudocapillarization is a significant age-related change in the baboon liver. Aging in baboons is associated with a novel aging change in the stellate cell not reported in other species. Hepatic pseudocapillarization is a widespread aging liver change found in several species including humans and other non-human primates.
背景/目的：在大鼠和人类中均已报道了肝血窦中与年龄相关的变化，即假毛细血管化，这对老年疾病易感性具有重要意义。在本研究中，我们调查了老年狒狒肝脏中是否发生类似变化，从而确定这是否代表一种广泛存在的衰老变化。
通过透射电子显微镜、扫描电子显微镜和免疫组织化学对5只年轻狒狒（5.4±0.5岁）和5只老年狒狒（21.8±0.7岁）的肝脏组织进行比较。
老年狒狒肝血窦内皮厚度增加（130±8纳米对186±9纳米，P<0.001），内皮窗孔频率降低，孔隙率从4.2±0.5%降至2.4±0.4%（P=0.006）。层粘连蛋白和血管性血友病因子在老年狒狒中的表达更为广泛。新型的窦周环形细胞，可能是脂肪充盈的星状细胞，在老年狒狒中较为突出。
假毛细血管化是狒狒肝脏中与年龄相关的显著变化。狒狒的衰老与星状细胞中一种其他物种未报道的新型衰老变化有关。肝假毛细血管化是在包括人类和其他非人类灵长类动物在内的多个物种中发现的广泛存在的肝脏衰老变化。
