Colling Lisa, Essmann Michael, Hollmer Cara, Larsen Bryan
Infectious Disease Research Laboratory, Des Moines University, Des Moines, IA 50312-4198, USA.
Infect Dis Obstet Gynecol. 2005 Sep;13(3):171-7. doi: 10.1080/10647440500068149.
To determine whether several topical compounds and other chemical entities are able to diminish the surface hydrophobicity of yeast cells.
Hydrophobicity of yeast cells was determined by binding styrene microspheres to the surface of untreated yeast or yeast pre-incubated with various substances with potential for cell surface modification. The degree of microsphere adherence to yeast cells was measured by flow cytometry.
A significant reduction in cell surface hydrophobicity was observed when yeast was incubated in protein-containing media. Other compounds that effectively reduced microsphere binding were various formulations of K-Y and heparin. Divalent cations (Ca+ + , Mg+ + , Zn+ + , Cu + + ) were also potent inhibitors of microsphere adherence. It was possible to remove substances contributing to microsphere binding by chemical extraction of the yeast. Yeast having reduced microsphere binding activity also showed diminished binding of concanavalin A.
Several commercially available compounds were able to block binding of styrene microspheres to yeast. Some of the binding activity appeared to be attributable to mannose-containing surface components. These findings have implications for formulating therapeutic products that might block yeast binding to tissues.
确定几种局部用化合物及其他化学物质是否能够降低酵母细胞的表面疏水性。
通过将苯乙烯微球结合到未处理的酵母或预先与各种具有细胞表面修饰潜力的物质孵育的酵母表面来测定酵母细胞的疏水性。通过流式细胞术测量微球对酵母细胞的粘附程度。
当酵母在含蛋白质的培养基中孵育时，观察到细胞表面疏水性显著降低。其他有效降低微球结合的化合物是各种配方的K-Y和肝素。二价阳离子（Ca²⁺、Mg²⁺、Zn²⁺、Cu²⁺）也是微球粘附的有效抑制剂。通过对酵母进行化学提取可以去除导致微球结合的物质。微球结合活性降低的酵母对伴刀豆球蛋白A的结合也减少。
几种市售化合物能够阻断苯乙烯微球与酵母的结合。部分结合活性似乎归因于含甘露糖的表面成分。这些发现对于开发可能阻断酵母与组织结合的治疗产品具有启示意义。
