Hazen B W, Hazen K C
Acadiana Medical Research Foundation, Lafayette, LA 70504-1007.
J Immunol Methods. 1988 Mar 16;107(2):157-63. doi: 10.1016/0022-1759(88)90214-1.
A simple method to assess fungal cell surface hydrophobicity involves enumeration of cell-attached, polystyrene latex microspheres. Modifications and conditions of this method for application to immune cell populations were investigated. The media used for suspending cells and microspheres appeared to influence microsphere attachment. Several tissue culture media supported high levels of microsphere attachment, but serum inhibited attachment. The concentration of microspheres also influenced the apparent level of detectable cell surface hydrophobicity. Under conditions which allow different levels of apparent cell surface hydrophobicity to be discriminated, the assay revealed that surface hydrophobicity of YAC-1 cells, which are used as standard targets in murine natural killer cell assays, varied depending on the time of harvest during growth and that phagocytic populations differed in cell surface hydrophobicity. Trypsinization experiments indicated that one hydrophobic constituent of the cell surface includes protein. These results indicate that the microsphere assay is a useful method for assessing cell surface hydrophobicity. The possibility that the assay could be used to determine quantitatively the surface free energies of immune cells and cell targets is discussed.
一种评估真菌细胞表面疏水性的简单方法是对附着在细胞上的聚苯乙烯乳胶微球进行计数。研究了将该方法应用于免疫细胞群体的改进方法和条件。用于悬浮细胞和微球的培养基似乎会影响微球的附着。几种组织培养基支持高水平的微球附着，但血清会抑制附着。微球的浓度也会影响可检测到的细胞表面疏水性的表观水平。在能够区分不同表观细胞表面疏水性水平的条件下，该测定法显示，在小鼠自然杀伤细胞测定中用作标准靶标的YAC-1细胞的表面疏水性会因生长过程中的收获时间而异，并且吞噬细胞群体的细胞表面疏水性也有所不同。胰蛋白酶消化实验表明，细胞表面的一种疏水成分包括蛋白质。这些结果表明，微球测定法是评估细胞表面疏水性的一种有用方法。文中还讨论了该测定法可用于定量测定免疫细胞和细胞靶标的表面自由能的可能性。
