Telekes András, Kiss-Tóth Endre, Nagy Tünde, Qwarnstrom Eva E, Kúsz Erzsébet, Polgár Timea, Resetár Akos, Dower Steven K, Duda Erno
Chemotherapy Day-Ward Unit, National Institute of Oncology, Ráth György u. 7-9, 1122 Budapest, Hungary.
Ann N Y Acad Sci. 2005 Jun;1051:515-28. doi: 10.1196/annals.1361.096.
Macrophages activated by lipopolysaccharide and/or phorbol esters exhibited high sensitivity to Avemar, a fermented wheat germ extract. Avemar synergized with lipopolysaccharide and PMA in the induction of the transcription of cytokine genes and release of inflammatory cytokines. At higher concentrations the preparation had a significant negative effect on the proliferation and survival of activated myeloid cell types. Avemar treatment induced the synthesis of ICAM-1 and synergized with the ICAM-inducing effect of TNF, but had no effect on VCAM-1 expression on microvascular endothelial cells. The effect of Avemar on signaling pathways, which are involved in cell activation was studied on HeLa cells as a model system. Avemar treatment increased the activity of stress kinases in a concentration-dependent way, resulting in the activation of AP-1 transcription factor. NF-kappa B-sensitive reporters were also activated by Avemar; in contrast, no effect of the preparation was observed on PKA-sensitive signaling pathways.
由脂多糖和/或佛波酯激活的巨噬细胞对Avemar（一种发酵的小麦胚芽提取物）表现出高度敏感性。Avemar与脂多糖和佛波酯协同作用，诱导细胞因子基因转录并释放炎性细胞因子。在较高浓度下，该制剂对活化的髓样细胞类型的增殖和存活有显著负面影响。Avemar处理诱导细胞间黏附分子-1（ICAM-1）的合成，并与肿瘤坏死因子（TNF）诱导ICAM的作用协同，但对微血管内皮细胞上血管细胞黏附分子-1（VCAM-1）的表达没有影响。以HeLa细胞作为模型系统，研究了Avemar对参与细胞活化的信号通路的影响。Avemar处理以浓度依赖的方式增加应激激酶的活性，导致活化蛋白-1（AP-1）转录因子的激活。NF-κB敏感报告基因也被Avemar激活；相反，未观察到该制剂对蛋白激酶A（PKA）敏感信号通路有影响。
