从金黄色葡萄球菌医院分离株CM.S2（IMCJ1454）中克隆并鉴定一种新型耐甲氧苄啶二氢叶酸还原酶
Cloning and characterization of a novel trimethoprim-resistant dihydrofolate reductase from a nosocomial isolate of Staphylococcus aureus CM.S2 (IMCJ1454).
作者信息
Sekiguchi Jun-ichiro, Tharavichitkul Prasit, Miyoshi-Akiyama Tohru, Chupia Vena, Fujino Tomoko, Araake Minako, Irie Atsushi, Morita Koji, Kuratsuji Tadatoshi, Kirikae Teruo
机构信息
Department of Infectious Diseases, Research Institute, International Medical Center of Japan, 1-21-1 Toyama, Shinjuku, Tokyo 162-8655, Japan.
出版信息
Antimicrob Agents Chemother. 2005 Sep;49(9):3948-51. doi: 10.1128/AAC.49.9.3948-3951.2005.
Abstract
A novel gene, dfrG, encoding a trimethoprim (TMP)-resistant dihydrofolate reductase (DHFR, designated S3DHFR) was cloned from a clinical isolate of methicillin-resistant Staphylococcus aureus. Escherichia coli expressing dfrG was highly resistant to TMP. Recombinant S3DHFR exhibited DHFR activity that was not inhibited by TMP.
摘要
从一株耐甲氧西林金黄色葡萄球菌临床分离株中克隆出一个新基因dfrG，其编码一种对甲氧苄啶（TMP）耐药的二氢叶酸还原酶（DHFR，命名为S3DHFR）。表达dfrG的大肠杆菌对TMP具有高度耐药性。重组S3DHFR表现出不受TMP抑制的DHFR活性。
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