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金黄色葡萄球菌ATCC 25923染色体上对甲氧苄啶敏感的二氢叶酸还原酶基因的特性分析

Characterization of the gene for chromosomal trimethoprim-sensitive dihydrofolate reductase of Staphylococcus aureus ATCC 25923.

作者信息

Dale G E, Then R L, Stüber D

机构信息

Department of New Technologies, F. Hoffmann-La Roche Ltd., Basel, Switzerland.

出版信息

Antimicrob Agents Chemother. 1993 Jul;37(7):1400-5. doi: 10.1128/AAC.37.7.1400.

DOI:10.1128/AAC.37.7.1400
PMID:8363365
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC187982/
Abstract

The gene for the trimethoprim-sensitive (Tmps) chromosomal dihydrofolate reductase (DHFR) of Staphylococcus aureus ATCC 25923 was cloned and characterized. The structural gene encodes a polypeptide of 159 amino acid residues and has a calculated molecular weight of 18,251. The amino acid sequences of this Tmps DHFR and those of the trimethoprim-resistant type S1 DHFR encoded by transposon Tn4003 are 80% identical. In contrast to the trimethoprim-resistant enzyme, the Tmps DHFR can be highly overexpressed in Escherichia coli, with most of the recombinant protein occurring in a soluble and an active form.

摘要

对金黄色葡萄球菌ATCC 25923的甲氧苄啶敏感（Tmps）染色体二氢叶酸还原酶（DHFR）基因进行了克隆和表征。该结构基因编码一个由159个氨基酸残基组成的多肽，计算分子量为18251。这种Tmps DHFR的氨基酸序列与转座子Tn4003编码的甲氧苄啶抗性S1型DHFR的氨基酸序列有80%的同一性。与甲氧苄啶抗性酶不同，Tmps DHFR在大肠杆菌中可高度过表达，大多数重组蛋白以可溶且有活性的形式存在。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e597/187982/e75bcb4585fe/aac00029-0031-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e597/187982/e75bcb4585fe/aac00029-0031-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e597/187982/e75bcb4585fe/aac00029-0031-a.jpg

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