Golubov Andrey, Gierczynski Rafal, Heesemann Jürgen, Rakin Alexander
Yersinia Group I, Max von Pettenkofer-Institute for Hygiene and Medical Microbiology, Pettenkofer Str. 9a, D-80336 Munich, Germany.
Int J Med Microbiol. 2005 Aug;295(4):213-26. doi: 10.1016/j.ijmm.2005.05.007.
The structure and distribution of IS Yen2, a new Yersinia enterocolitica insertion sequence element, were investigated. ISYen2 is related to IS elements of the IS21 family and is present in two isoforms in Y. enterocolitica serotype O:3. Analysis of all copies of both isoforms, IS Yen2A and IS Yen2B, by PCR and sequencing indicated that they are not flanked by direct repeats which are typical of the IS21 family of repetitive elements. IS Yen2 is present in multiple copies in Y. enterocolitica O:3 and O:9 and in a single copy in Y. enterocolitica O:1 and O:2 serotypes. The probe for IS Yen2 efficiently detected all weakly pathogenic Y. enterocolitica bioserotypes investigated, whereas it did not hybridize with other strains. This indicates that IS Yen2 can serve as an additional tool for Y. enterocolitica differentiation and epidemiological studies. Distribution of the different groups of IS elements in two Y. enterocolitica pathotypes is in favor of the parallel evolution of American and European Y. enterocolitica strains.
对一种新的小肠结肠炎耶尔森氏菌插入序列元件IS Yen2的结构和分布进行了研究。ISYen2与IS21家族的IS元件相关，并且在小肠结肠炎耶尔森氏菌血清型O:3中以两种异构体形式存在。通过PCR和测序对两种异构体IS Yen2A和IS Yen2B的所有拷贝进行分析表明，它们两侧没有重复元件IS21家族典型的同向重复序列。IS Yen2在小肠结肠炎耶尔森氏菌O:3和O:9中以多拷贝形式存在，而在小肠结肠炎耶尔森氏菌O:1和O:2血清型中以单拷贝形式存在。IS Yen2探针能有效检测所研究的所有弱致病性小肠结肠炎耶尔森氏菌生物血清型，而与其他菌株不杂交。这表明IS Yen2可作为小肠结肠炎耶尔森氏菌鉴别和流行病学研究的一种额外工具。两种小肠结肠炎耶尔森氏菌致病型中不同IS元件组的分布有利于美国和欧洲小肠结肠炎耶尔森氏菌菌株的平行进化。
