Espinosa E, Vara J A Fresno, Redondo A, Sánchez J J, Hardisson D, Zamora P, Pastrana F Gómez, Cejas P, Martínez B, Suárez A, Calero F, Barón M González
Service of Medical Oncology, Hospital La Paz, P de la Castellana, 261--28046 Madrid, Spain.
J Clin Oncol. 2005 Oct 10;23(29):7278-85. doi: 10.1200/JCO.2005.01.4746. Epub 2005 Aug 29.
We sought to reproduce with quantitative reverse-transcriptase polymerase chain reaction (qRT-PCR) the results obtained with a 70-gene expression profile that has been described previously in breast cancer.
Frozen breast cancer samples from patients who were operated on were used to isolate tumor RNA. Ninety-six patients with stage I to II disease were included. Median age was 57 years (range, 27 to 80 years). Forty-eight patients had lymph node-negative and 48 lymph node-positive disease. qRT-PCR amplifications were performed and the results were correlated with clinical data.
After a minimum follow-up of 5 years, 25 patients had a relapse. The gene profile divided patients into two groups with poor and good prognosis. Significant differences with regard to grade of differentiation, size and hormone receptors were seen between the two groups. The gene profile was significantly associated with relapse-free survival and overall survival in the whole group of 96 patients. Multivariate analysis showed that only lymph node status and gene profile were significantly correlated to overall survival.
qRT-PCR reproduced the results obtained with microarrays for a prognostic gene profile in women with early-stage breast cancer.
我们试图通过定量逆转录聚合酶链反应（qRT-PCR）重现先前在乳腺癌中描述的70基因表达谱所获得的结果。
使用接受手术治疗患者的冷冻乳腺癌样本分离肿瘤RNA。纳入96例I至II期疾病患者。中位年龄为57岁（范围27至80岁）。48例患者为淋巴结阴性疾病，48例为淋巴结阳性疾病。进行qRT-PCR扩增，并将结果与临床数据相关联。
经过至少5年的随访，25例患者出现复发。基因谱将患者分为预后不良和良好的两组。两组在分化程度、大小和激素受体方面存在显著差异。基因谱与96例患者的总无复发生存率和总生存率显著相关。多变量分析表明，只有淋巴结状态和基因谱与总生存率显著相关。
qRT-PCR重现了微阵列在早期乳腺癌女性预后基因谱方面所获得的结果。
